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dc.contributor.authorWissuwa, Juliane
dc.contributor.authorStokke, Runar
dc.contributor.authorFedøy, Anita-Elin
dc.contributor.authorLian, Kjersti
dc.contributor.authorSmalås, Arne O.
dc.contributor.authorSteen, Ida Helene
dc.date.accessioned2017-03-21T15:05:32Z
dc.date.available2017-03-21T15:05:32Z
dc.date.issued2016-02-24
dc.description.abstractMembers of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.en_US
dc.descriptionPublished version. Source at http://doi.org/10.1186/s40793-016-0137-y. License CC BY 4.0.en_US
dc.identifier.citationWissuwa J, Stokke R, Fedøy A, Lian K, Smalås A, Steen IH. Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site. Standards in Genomic Sciences. 2016;11:16:1-12en_US
dc.identifier.cristinIDFRIDAID 1341826
dc.identifier.doi10.1186/s40793-016-0137-y
dc.identifier.issn1944-3277
dc.identifier.urihttps://hdl.handle.net/10037/10810
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.journalStandards in Genomic Sciences
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/208491/Norway/Mining of a Norwegian biogoldmine through metagenomics//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/221568/Norway/Enzyme development for Norwegian biomass - mining Norwegian biodiversity for seizing Norwegian opportunities in the bio-based economy//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/SFF/179560/NorwayProjectNumber/CGB Senter for geobiologi dyphav, dyp biosfære, dyp tid & livets røtter//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subjectThermophileen_US
dc.subjectGeobacillusen_US
dc.subjectEnzymesen_US
dc.subjectBioprospectingen_US
dc.titleIsolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent siteen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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