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dc.contributor.authorBerg, Kristel
dc.contributor.authorLeiros, Ingar
dc.contributor.authorWilliamson, Adele Kim
dc.date.accessioned2020-04-08T09:32:05Z
dc.date.available2020-04-08T09:32:05Z
dc.date.issued2019-03-02
dc.description.abstractDNA ligases operating at low temperatures have potential advantages for use in biotechnological applications. For this reason, we have characterized the temperature optima and thermal stabilities of three minimal Lig E-type ATP-dependent DNA ligase originating from Gram-negative obligate psychrophilic bacteria. The three ligases, denoted Vib-Lig, Psy-Lig, and Par-Lig, show a remarkable range of thermal stabilities and optima, with the first bearing all the hallmarks of a genuinely cold-adapted enzyme, while the latter two have activity and stability profiles more typical of mesophilic proteins. A comparative approach based on sequence comparison and homology modeling indicates that the cold-adapted features of Vib-Lig may be ascribed to differences in surface charge rather than increased local or global flexibility which is consistent with the contemporary emerging paradigm of the physical basis of cold adaptation of enzymes.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in <i>Extremophiles</i>. The final authenticated version is available online at: https://doi.org/10.1007/s00792-019-01082-y.en_US
dc.identifier.citationBerg K, Leiros I, Williamson AK. Temperature adaptation of DNA ligases from psychrophilic organisms. Extremophiles. 2019;23(3):305-317en_US
dc.identifier.cristinIDFRIDAID 1714694
dc.identifier.doi10.1007/s00792-019-01082-y
dc.identifier.issn1431-0651
dc.identifier.issn1433-4909
dc.identifier.urihttps://hdl.handle.net/10037/18024
dc.language.isoengen_US
dc.publisherSpringer Verlagen_US
dc.relation.journalExtremophiles
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/244247/Norway/Engineering efficient DNA ligases for improved Next-Generation-Sequencing//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© Springer Japan KK, part of Springer Nature 2019en_US
dc.subjectVDP::Mathematics and natural science: 400::Chemistry: 440en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Kjemi: 440en_US
dc.titleTemperature adaptation of DNA ligases from psychrophilic organismsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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