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dc.contributor.authorOrefice, Ida
dc.contributor.authorGerecht, Andrea Cornelia
dc.contributor.authord'Ippolito, G
dc.contributor.authorFontana, Angelo
dc.contributor.authorIanora, Adrianna
dc.contributor.authorRomano, Giovanna
dc.date.accessioned2022-04-20T07:47:42Z
dc.date.available2022-04-20T07:47:42Z
dc.date.issued2015-09-11
dc.description.abstractEcologically-relevant marine diatoms produce a plethora of bioactive oxylipins deriving from fatty acid oxidation, including aldehydes, hydroxy-fatty acids, epoxy-hydroxy-fatty acids, and oxo-acids. These secondary metabolites have been related to the negative effect of diatoms on copepod reproduction, causing low hatching success and teratogenesis in the offspring during periods of intense diatom blooms. The common intermediates in the formation of oxylipins are fatty acid hydroperoxides. The quantitative measurement of these intermediates can fundamentally contribute to understanding the function and role of lipoxygenase metabolites in diatom-copepod interactions. Here, we describe the successful adaptation of the ferrous oxidation-xylenol orange 2 (FOX2) assay to diatom samples, which showed several advantages over other spectrophotometric and polarographic methods tested in the present work. Using this method we assessed fatty acid hydroperoxide levels in three diatom species: Skeletonema marinoi, Thalassiosira rotula, and Chaetoceros affinis, and discuss results in light of the literature data on their detrimental effects on copepod reproduction.en_US
dc.identifier.citationOrefice, Gerecht AC, d'Ippolito G, Fontana A, Ianora A, Romano G. Determination of Lipid Hydroperoxides in Marine Diatoms by the FOX2 Assay. Marine Drugs. 2015;13(9):5767-5783en_US
dc.identifier.cristinIDFRIDAID 1328396
dc.identifier.doi10.3390/md13095767
dc.identifier.issn1660-3397
dc.identifier.urihttps://hdl.handle.net/10037/24811
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalMarine Drugs
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2015 The Author(s)en_US
dc.titleDetermination of Lipid Hydroperoxides in Marine Diatoms by the FOX2 Assayen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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