Show simple item record

dc.contributor.authorOlsen, Stian
dc.contributor.authorKetelsen Striberny, Bernd
dc.contributor.authorHollmann, Julien
dc.contributor.authorSchwacke, Rainer
dc.contributor.authorPopper, Zoe A.
dc.contributor.authorKrause, Kirsten
dc.date.accessioned2022-04-27T10:52:13Z
dc.date.available2022-04-27T10:52:13Z
dc.date.issued2015-11-11
dc.description.abstractChanges in cell walls have been previously observed in the mature infection organ, or haustorium, of the parasitic angiosperm Cuscuta, but are not equally well charted in young haustoria. In this study, we focused on the molecular processes in the early stages of developing haustoria; that is, before the parasite engages in a physiological contact with its host. We describe first the identification of differentially expressed genes in young haustoria whose development was induced by far-red light and tactile stimuli in the absence of a host plant by suppression subtractive hybridization. To improve sequence information and to aid in the identification of the obtained candidates, reference transcriptomes derived from two species of Cuscuta, C. gronovii and C. reflexa, were generated. Subsequent quantitative gene expression analysis with different tissues of C. reflexa revealed that among the genes that were up-regulated in young haustoria, two xyloglucan endotransglucosylase/hydrolase (XTH) genes were highly expressed almost exclusively at the onset of haustorium development. The same expression pattern was also found for the closest XTH homologues from C. gronovii. In situ assays for XTH-specific action suggested that xyloglucan endotransglucosylation was most pronounced in the cell walls of the swelling area of the haustorium facing the host plant, but was also detectable in later stages of haustoriogenesis. We propose that xyloglucan remodelling by Cuscuta XTHs prepares the parasite for host infection and possibly aids the invasive growth of the haustorium.en_US
dc.identifier.citationOlsen S, Ketelsen Striberny B, Hollmann, Schwacke R, Popper ZA, Krause K. Getting ready for host invasion: Elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscuta. Journal of Experimental Botany. 2016;67(3):695-708en_US
dc.identifier.cristinIDFRIDAID 1310904
dc.identifier.doi10.1093/jxb/erv482
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.urihttps://hdl.handle.net/10037/24909
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.journalJournal of Experimental Botany
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2016 The Author(s)en_US
dc.titleGetting ready for host invasion: Elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscutaen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record