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dc.contributor.authorOpstad, Ida Sundvor
dc.contributor.authorGodtliebsen, Gustav
dc.contributor.authorStröhl, Florian
dc.contributor.authorMyrmel, Truls
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorAgarwal, Krishna
dc.contributor.authorBirgisdottir, Åsa birna
dc.date.accessioned2022-08-26T09:32:40Z
dc.date.available2022-08-26T09:32:40Z
dc.date.issued2022-03-23
dc.description.abstractThis three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM to investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts in living or fxed cells. MDVs act as a mitochondria quality control mechanism. The cells were stably expressing the tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used as a sensor for acidity. A part of the dataset is showing correlative imaging of lysosomes labeled using LysoTracker in fxed and living cells. The cells were cultivated in either normal or glucose-deprived medium containing galactose. The resulting 3DSIM data were of high quality and can be used to undertake a variety of studies. Interestingly, many dynamic tubules derived from mitochondria are visible in the 3DSIM videos under both glucose and galactose-adapted growth conditions. As the raw 3DSIM data, optical parameters, and reconstructed 3DSIM images are provided, the data is especially suitable for use in the development of SIM reconstruction algorithms, bioimage analysis methods, and for biological studies of mitochondria.en_US
dc.identifier.citationOpstad IS, Godtliebsen G, Ströhl F, Myrmel T, Ahluwalia BS, Agarwal K, Birgisdottir Åb. Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions. Scientific Data. 2022;9(1)en_US
dc.identifier.cristinIDFRIDAID 2014223
dc.identifier.doi10.1038/s41597-022-01207-7
dc.identifier.issn2052-4463
dc.identifier.urihttps://hdl.handle.net/10037/26437
dc.language.isoengen_US
dc.publisherNatureen_US
dc.relation.journalScientific Data
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleThree-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditionsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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