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dc.contributor.authorStröhl, Florian
dc.contributor.authorHansen, Daniel Henry
dc.contributor.authorNager Grifo, Mireia
dc.contributor.authorBirgisdottir, Åsa Birna
dc.date.accessioned2022-10-12T11:26:00Z
dc.date.available2022-10-12T11:26:00Z
dc.date.issued2022
dc.description.abstractMultifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates optical sectioning and offers axial superresolution capabilities. In our method, a dithered oblique light-sheet scans the sample volume during a single exposure, while generated fluorescence is linearised onto the camera with a multifocus optical element. A synchronised rolling shutter readout realised optical sectioning. We describe the technique theoretically and verify its optical sectioning and superresolution capabilities. We demonstrate a prototype system with a multifocus beam splitter cascade and record monolayers of endothelial cells at 35 volumes per second. We furthermore image uncleared engineered human heart tissue and visualise the distribution of mitochondria at axial superresolution. Our method manages to capture sub-diffraction sized mitochondria-derived vesicles up to 30 um deep into the tissue.en_US
dc.identifier.citationStröhl, Hansen, Nager Grifo, Birgisdottir. Multifocus microscopy with optically sectioned axial superresolution. arXiv.org. 2022en_US
dc.identifier.cristinIDFRIDAID 2031370
dc.identifier.doi10.48550/arXiv.2206.01257
dc.identifier.issn2331-8422
dc.identifier.urihttps://hdl.handle.net/10037/27025
dc.language.isoengen_US
dc.relation.journalarXiv.org
dc.relation.projectIDNorges forskningsråd: 314546en_US
dc.relation.projectIDEC/H2020: 964800en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/OrganVision/964800/Norway/OrganVision: Technology for real-time visualizing and modelling of fundamental process in living organoids towards new insights into organ-specific health, disease, and recovery/EXELLENT SCIENCE/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleMultifocus microscopy with optically sectioned axial superresolutionen_US
dc.type.versionsubmittedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US


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