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dc.contributor.authorAssefa, Netsanet Gizaw
dc.contributor.authorHansen, Hilde
dc.contributor.authorAltermark, Bjørn
dc.date.accessioned2022-12-02T12:42:44Z
dc.date.available2022-12-02T12:42:44Z
dc.date.issued2022-05-06
dc.description.abstractPolyhydroxyalkanoates (PHAs) are natural biodegradable polyesters that are produced by numerous prokaryotic microorganisms primarily as a carbon- and energy reserve. The PhaC enzyme catalyzes the last step in the PHA biosynthesis pathway and synthesizes PHA polymers from hydroxyalkanoic acids. A type I PhaC from a PHAproducing marine bacterium Brevundimonas sp. KH11J01 (BrPhaC) was identified, produced recombinantly and characterized. Its properties were compared with its homolog from C. necator H16 (RePhaC). Unlike other PhaCs, it was found that BrPhaC is a lag-phase free enzyme organized as a trimer, even without the presence of a substrate. The enzymatic reaction is initiated instantly irrespective of temperature, in contrast to RePhaC in which the duration of the lag-phase was highly affected by temperature. At 10 ◦C BrPhaC was 40% active whereas RePhaC was barely active. The significance of using marine microorganisms, harboring cold-active PHA biosynthesis enzymes, for energy efficient PHA production, is also discussed briefly. The unique trimeric organization of BrPhaC challenges our understanding of the PhaC reaction mechanisms, which is mainly based on the crystal structures of the inactive forms of the enzyme.en_US
dc.identifier.citationAssefa, Hansen, Altermark. A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16. New Biotechnology. 2022;70:57-66en_US
dc.identifier.cristinIDFRIDAID 2060494
dc.identifier.doi10.1016/j.nbt.2022.05.003
dc.identifier.issn1871-6784
dc.identifier.issn1876-4347
dc.identifier.urihttps://hdl.handle.net/10037/27674
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalNew Biotechnology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleA unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)