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dc.contributor.authorSommernes, Jon-Richard
dc.contributor.authorMillett-Sikking, Alfred
dc.contributor.authorStröhl, Florian
dc.date.accessioned2024-09-09T11:13:50Z
dc.date.available2024-09-09T11:13:50Z
dc.date.issued2024-02-12
dc.description.abstractOblique plane microscopy (OPM) offers 3D optically sectioned imaging with high spatial- and temporal-resolution while enabling conventional sample mounting. The technique uses a concatenation of three microscopes, two for remote focusing and a tilted tertiary microscope, often including an immersion objective, to image an oblique sample plane. This design induces Fresnel reflections and a reduced effective aperture, thus impacting the resolution and light efficiency of the system. Using vectorial diffraction simulations, the system performance was characterized based on illumination angle and polarization, signal to noise ratio, and refractive index of the tertiary objective immersion. We show that for samples with high fuorescent anisotropy, s-polarized light-sheets yield higher average resolution for all system configurations, as well as higher light-efficiency. We also provide a tool for performance characterization of arbitrary light-sheet imaging systems.en_US
dc.identifier.citationSommernes, Millett-Sikking, Ströhl. S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy. Scientific Reports. 2024;14(1)en_US
dc.identifier.cristinIDFRIDAID 2245883
dc.identifier.doi10.1038/s41598-024-53900-9
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/34566
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalScientific Reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleS-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)