dc.contributor.author | Iliev, Dimitar B. | |
dc.contributor.author | Strandskog, Guro | |
dc.contributor.author | Sobhkhez, Mehrdad | |
dc.contributor.author | Bruun, Jack A. | |
dc.contributor.author | Jørgensen, Jorunn B. | |
dc.date.accessioned | 2022-02-11T10:02:39Z | |
dc.date.available | 2022-02-11T10:02:39Z | |
dc.date.issued | 2021-11-30 | |
dc.description.abstract | b‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in
vertebrate organisms. Being recognized by phagocytic leukocytes including
macrophages and neutrophils through receptors such as dectin-1 and Complement
receptor 3 (CR3), the BG are perceived by the innate immune system of vertebrates as
foreign substances known as Pathogen Associated Molecular Patterns (PAMPs). The
yeast-derived BG has been recognized for its potent biological activity and it is used as an
immunomodulator in human and veterinary medicine. The goal of the current study was to
characterize the immunostimulatory activity of soluble yeast BG in primary cultures of
Atlantic salmon (Salmo salar) head kidney leukocytes (HKLs) in which phagocytic cell
types including neutrophils and mononuclear phagocytes predominate. The effect of BG
on the secretome of HKL cultures, including secretion of extracellular vesicles (EVs) and
soluble protein55s was characterized through western blotting and mass spectrometry.
The results demonstrate that, along with upregulation of proinflammatory genes, BG
induces secretion of ubiquitinated proteins (UbP), MHCII-containing EVs from professional
antigen presenting cells as well as proteins derived from granules of polymorphonuclear
granulocytes (PMN). Among the most abundant proteins identified in BG-induced EVs
were beta-2 integrin subunits, including CD18 and CD11 homologs, which highlights the
role of salmon granulocytes and mononuclear phagocytes in the response to soluble BG.
Overall, the current work advances the knowledge about the immunostimulatory activity of
yeast BG on the salmon immune system by shedding light on the effect of this PAMP on
the secretome of salmon leukocytes. | en_US |
dc.identifier.citation | Iliev, Strandskog, Sobhkhez, Bruun, Jørgensen. Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan. Frontiers in Immunology. 2021;12:1-14 | en_US |
dc.identifier.cristinID | FRIDAID 1972582 | |
dc.identifier.doi | 10.3389/fimmu.2021.736964 | |
dc.identifier.issn | 1664-3224 | |
dc.identifier.uri | https://hdl.handle.net/10037/24014 | |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.relation.journal | Frontiers in Immunology | |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FRIMEDBIO/230735/Norway/Exosomes from salmon (Salmo salar) leukocytes - composition, functions, biogenesis and immunodiagnostic potential// | en_US |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
dc.title | Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |