Antibacterial activity in fish mucus from farmed fish
Permanent lenke
https://hdl.handle.net/10037/295Dato
2005-06-17Type
Master thesisMastergradsoppgave
Forfatter
Mozumder, Mohammad Mojibul HoqueSammendrag
Mucus samples were collected from five different farmed Atlantic salmon (Salmo salar)
and Atlantic cod (Gadus morhua) in order to study the content of antibacterial activity in
the mucus and to compare the activity of mucus samples of the two different fish species.
The mucus samples were diluted in buffer, desalted and adjusted to different protein
concentrations in the mucus samples, A search for antibacterial activity in the mucus was
performed for different protein concentrations, against four different bacterial strains
Escherichia coli, Listionella anguillarum, Corynebacterrium glutamicum and
Staphylococcus aureus.Protein concentrations in cod mucus samples were found higher
than the salmon mucus samples when tested by different protein assays, Bicinchonic
assay (BCA) and absorbance based method with measurement in spectrophotometer at
A280 nm. Antibacterial activity was detected in mucus samples of both fish species,
against the four bacterial strains. The bacteria C. glutamicum was the most sensitive,
while S. aureus was the least sensitive against the different mucus samples. Furthermore,
mucus samples of cod contained more antibacterial active substances than the mucus
samples of the salmon. The results of this study indicate that that the mucus of salmon
and cod contain one or several components with antibacterial activity. These bioactive
substances may play an important role in the ability of mucus to defend salmon and cod
against pathogenic bacteria or microorganisms. However, more investigation is necessary
to confirm the antibacterial activity in cod and salmon mucus and to purify and
characterize the active components.
Forlag
Universitetet i TromsøUniversity of Tromsø
Metadata
Vis full innførselSamlinger
Copyright 2005 The Author(s)
Følgende lisensfil er knyttet til denne innførselen: