A mesoscopic axially swept oblique plane microscope for imaging of freely moving organisms with near-isotropic resolution
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https://hdl.handle.net/10037/34926Date
2024-08-09Type
Journal articleTidsskriftartikkel
Author
Prevedel, Robert; Davis, Samuel; Sommernes, Jon-Richard; hambura, sebastian; Riedel, Levin; Gil, Alejandro; Ikmi, Aissam; Ströhl, FlorianAbstract
Rapid three-dimensional imaging over extended fields of views (FOVs) is crucial to the study of organism-wide systems and biological processes in vivo. Selective-plane illumination microscopy (SPIM) is a powerful method for high spatio-temporal resolution in toto imaging of such biological specimens. However, typical SPIM implementations preclude conventional sample mounting and have anisotropic imaging performance, in particular when designed for large FOVs over 1 mm diameter. Here, we introduce axial sweeping of the illumination into a non-orthogonal dual-objective oblique plane microscope (OPM) design, thereby enabling the observation of freely moving animals over millimeter sized FOVs, at close to isotropic, sub-cellular resolution. We apply our mesoscopic axially swept OPM (MASOPM) to imaging the behavioral dynamics of the sea anemone Nematostella vectensis over 1 × 0.7 × 0.4 mm at 1.7 × 2.6 × 3.7 µm resolution and 0.5 Hz volume rate.
Publisher
Optica Publishing GroupCitation
Prevedel, Davis, Sommernes, hambura, Riedel, Gil, Ikmi, Ströhl. A mesoscopic axially swept oblique plane microscope for imaging of freely moving organisms with near-isotropic resolution. Optica Open. 2024Metadata
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