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dc.contributor.authorEdema, Hilary
dc.contributor.authorBawin, Thomas Georges A
dc.contributor.authorOlsen, Stian
dc.contributor.authorKrause, Kirsten
dc.contributor.authorKarppinen, Katja Hannele
dc.date.accessioned2024-10-08T06:55:41Z
dc.date.available2024-10-08T06:55:41Z
dc.date.issued2024-04-17
dc.description.abstractCuscuta campestris is a common and problematic parasitic plant which relies on haustoria to connect to and siphon nutrients from host plants. Glycoside hydrolase family 9 (GH9) cellulases (EC 3.2.1.4) play critical roles in plant cell wall biosynthesis and disassembly, but their roles during Cuscuta host invasion remains underexplored. In this study, we identified 22 full-length GH9 cellulase genes in C. campestris genome, which encoded fifteen secreted and seven membrane-anchored cellulases that showed distinct phylogenetic relationships. Expression profiles suggested that some of the genes are involved in biosynthesis and remodeling of the parasite’s cell wall during haustoriogenesis, while other genes encoding secreted B- and C-type cellulases are tentatively associated with degrading host cell walls during invasion. Transcriptomic data in a host-free system and in the presence of susceptible or partially resistant tomato hosts, showed for especially GH9B7, GH9B11 and GH9B12 a shift in expression profiles in the presence of hosts, being more highly expressed during host attachment, indicating that Cuscuta can tune cellulase expression in response to a host. Functional analyses of recombinant B- and C-type cellulases showed endoglucanase activities over wide pH and temperature conditions, and activities towards multiple cellulose and hemicellulose substrates. These findings improve our understanding of host cell wall disassembly by Cuscuta, and cellulase activity towards broad substrate range potentially explain its wide host range. This is the first study to provide a broad biochemical insight into Cuscuta GH9 cellulases, which based on our study may have potential applications in industrial bioprocessing.en_US
dc.identifier.citationEdema, Bawin, Olsen, Krause, Karppinen. Parasitic dodder expresses an arsenal of secreted cellulases with multi-substrate specificity during host invasion. Plant physiology and biochemistry (Paris). 2024;210en_US
dc.identifier.cristinIDFRIDAID 2267188
dc.identifier.doi10.1016/j.plaphy.2024.108633
dc.identifier.issn0981-9428
dc.identifier.issn1873-2690
dc.identifier.urihttps://hdl.handle.net/10037/35105
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalPlant physiology and biochemistry (Paris)
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleParasitic dodder expresses an arsenal of secreted cellulases with multi-substrate specificity during host invasionen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)