Mastergradsoppgaver i marin bioteknologi: Recent submissions
Now showing items 21-40 of 45
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Antibiotic resistance profiles and polystyrene biofilm formation capabilities of bacteria isolated from wastewater in Tromsø
(Master thesis; Mastergradsoppgave, 2021-05-18)Pollution of plastic in itself has become a growing focus in the last 15 years. In addition, the impact of plastic as a reservoir for pathogenic bacteria and transfer of antibiotic resistance genes has become a global concern, for individuals, organizations, policymakers and health organizations. It is important to document the relationship of microbiota associated to different types of plastic ... -
Genomic characterization and insights of local adaptation in Norwegian juvenile lumpfish populations
(Master thesis; Mastergradsoppgave, 2021-05-18)The lumpfish (Cyclopterus lumpus Linnaeus, 1758) has for many years been an attractive target for the roe fisheries in Norway and has more recently become an important cleanerfish in the salmonid farming industry for the control of salmon lice (Copepoda: Caligidae). Despite this, there is a lack of knowledge about several life aspects of the species and studies on its genetic structure have been ... -
A Comparison of Biochemical Composition and Bioactivity of Abalone (Haliotis spp) Subjected to Different Diets and Treatments
(Master thesis; Mastergradsoppgave, 2021-03-01)The fast growth of the aquaculture sector has led to an unsustainable harvest of wild fish species used in the production of aquaculture feed. Integrated Multi-trophic Aquaculture (IMTA) promises ecological and socio-economic advantages that include the provision of sustainable aquaculture feed. However, the promotion of IMTA requires that the stakeholders have sufficient quantitative information ... -
Biodiscovery of Novel RNA Ligases with Biotechnological Potential - New Insights into the RNA Ligase 3 Family
(Master thesis; Mastergradsoppgave, 2020-08-04)Polynucleotide ligases constitute a class of enzymes which catalyse the formation of phosphodiester bonds between proximal 5’-PO4 and 3’-OH groups in DNA, RNA and DNA/RNA-hybrid substrates. The reaction proceeds through three steps, where steps 1 and 2 prepare the substrate for ligation in step 3. The focus of this thesis is on ssRNA ligases of the RNA ligase (Rnl) 3 family. RNA ligases can repair ... -
The Role of Silicate in the Parasitism of Pleurosigma sp. Diatoms by the Thraustochytrid Phycophthorum isakeiti
(Master thesis; Mastergradsoppgave, 2020-05-15)The recently described thraustochytrid diatom parasite Phycophthorum isakeiti has been isolated from the marine system of northern Norway (Hassett, 2020). Protist P. isakeiti’s ecological role, biodiscovery potential, and interactions with a possible diatom host, Pleurosigma sp. remain largely unexplored. Here I present culturing experiments to test whether P. isakeiti is an obligate parasite. To ... -
CO2 i sjø og på land. En lokal studie med fokus på fabrikkrøyk og miljø.
(Master thesis; Mastergradsoppgave, 2019-11-15)Netto utslipp av karbon til atmosfæren har økt siden starten på den industrielle revolusjon. Rundt halvparten av karbon som slippes ut blir tatt opp av havet fysisk via karbon fluks eller bundet opp i økosystemer i havet eller på land. Denne oppgaven tolker og diskuterer potensielle lokale virkninger av industriell CO2 utslipp ved å benytte fysiske og biologiske, oseanografi og CO2 data samlet ... -
Expression of Secondary Metabolite Gene Clusters and Production of Secondary Metabolites in Three Nostoc strains Subjected to Deprivation on Nutrients and Competition
(Master thesis; Mastergradsoppgave, 2019-05-15)Cyanobacteria are a unique source of natural products, where most of them are synthesized by non-ribosomal peptide synthase, polyketide synthase, or a hybrid of these pathways. The identification of such biosynthetic genes responsible for the production of secondary metabolites is still a relatively unexplored area, and it remains many natural products for which a biosynthetic origin is unknown. ... -
Mikrobiota hos grønn kråkebolle, Strongylocentrotus droebachiensis. Fokus på identifikasjon av bakteriestammer
(Master thesis; Mastergradsoppgave, 2017-05-15)It has been reported that the green sea urchin, Strongylocentrotus droebachiensis, can be affected by symptoms that is associated with the disease called “bald sea urchin disease”. However, no pathogen for sea urchins are known in the literature, but a working hypothesis claims that species of the Vibrio splendidus clade can be associated with symptoms characterized as bald sea urchin disease in S. ... -
Isolering av etylester og triacylglyserol ved hjelp av fast fase ekstraksjon
(Master thesis; Mastergradsoppgave, 2018-05-15)I de siste par tiårene har det vært en kraftig økning i humant bruk av kosttilskudd som inneholder langkjedede omega-3 fettsyrer, det vil si eicosapentaensyre (EPA) og docosaheksaensyre (DHA). Årsaken er de antatte positive helseeffekter disse fettsyrene har. I dag er det vanligst at slike kosttilskudd tas i form av kapsler, blant annet fordi mange mennesker synes marine oljer er ubehagelig å drikke ... -
Bioactivity Potential of an Arctic Marine Diatom Species Cultivated at Different Conditions
(Master thesis; Mastergradsoppgave, 2018-05-15)Microalgae have proven to contain a vast amount of beneficial, high value compounds like proteins, lipids and powerful antioxidants as well as some interesting bioactive compounds. Nevertheless, microalgae are severely underrepresented in conjunction with marine bioactive natural product discovery. This thesis aims to unlock bioactivity potential of a microalgae species from the most abundant and ... -
Protein og kitin i grakse fra produksjon av Calanus® Oil
(Master thesis; Mastergradsoppgave, 2017-05-15)Letingen etter nye ressurser som er rik på flerumettede langkjedede omega-3 fettsyrer, har ført til utnyttelse av dyreplanktonet Calanus finmarchicus (raudåte). Det blir høstet inn ved tråling i overflaten under de mest lipidrike stadiene i vår- og sommermånedene. Den største andelen av lipidene er voksestere med et høyt innhold av omega-3 fettsyrer; stearidonsyre (18:4n-4), EPA (20:5n-3) og DHA ... -
Biokjemiske egenskaper til grakse fra produksjon av Calanus® Oil
(Master thesis; Mastergradsoppgave, 2017-05-15)Det er et stort behov for nye marine lipidkilder med et høyt innhold av LC-PUFA. Den store biomasseproduksjonen av raudåte (Calanus finmarchicus) i Norskehavet gjør den unik. Fra raudåte utvinner firmaet Calanus AS den kommersielle oljen Calanus® Oil. Gjennom prosessen dannes også to andre produkter, et proteinhydrolysat og en grakse (presskake). Det overordnede målet med masteroppgaven var å ... -
Isolation and characterisation of secondary metabolites from arctic, marine invertebrates
(Master thesis; Mastergradsoppgave, 2017-05-15)Bioprospecting is the systematic search for and discovery of products in nature, with the purpose of developing commercial products. The marine environment displays a rich biological diversity, as well as a diversity within environmental factors. This environment has necessitated the production of potent secondary metabolites by marine organisms in their arms race against predators and pathogens, ... -
Exploring the potential of two arctic marine bacteria for the production of bioactive metabolites
(Master thesis; Mastergradsoppgave, 2017-05-15)The number of compounds being isolated from the marine environment is increasing, and there is a great potential for discovering new marine derived drug candidates. Improved collection techniques has strengthened bioprospecting on a wider diversity of marine microorganisms. The focus on microorganisms has led to the realisation that many of the natural products originally isolated from macroorganisms, ... -
Exploring the antibacterial and anticancer potential of five marine fungi. With the use of OSMAC-approach
(Master thesis; Mastergradsoppgave, 2017-05-15)The marine environment is an untapped source for biodiversity and has a great potential to provide the drugs of the future. Antibiotic resistance is an increasing threat worldwide and the need for discovering new antibacterial compounds is urgent. Marine microorganisms produce a wide range of bioactive compounds, and marine fungi have only been exploited to a small extent. This creates a great ... -
Mikrobiell stabilitet og kjemisk sammensetning i hydrolysat fra Calanus finmarchicus
(Master thesis; Mastergradsoppgave, 2015-05-15)Calanus finmarchicus (raudåte) er den mest tallrike av alle marine skalldyrarter, med en stående bestand på cirka 70-80 millioner tonn i det Nordiske hav. Siden ny bærekraftig innhøstingsteknologi er utviklet for arten, er det mulig å utnytte denne ressursen kommersielt. I dag produseres olje fra C. finmarchicus, og har flere dokumenterte helseeffekter. Ved produksjon framstilles fire ganger mer ... -
Antimikrobielle peptider i O-skjell, Modiolus modiolus
(Master thesis; Mastergradsoppgave, 2015-05-29)I en tidligere studie utført på O-skjell (Modiolus modiolus) har antimikrobiell aktivitet blitt påvist i en rekke vevekstrakter, deriblant fra hemocyttene. En videreføring av dette arbeidet har ført til isoleringen av flere antimikrobielle peptider samt karakterisering av partielle N-terminale sekvenser. Dette masterprosjektet har hatt som hensikt å følge opp disse funnene ytterligere ved å påvise ... -
Effekter på bakteriesamfunn av boreslamdeponering på havbunnen
(Master thesis; Mastergradsoppgave, 2014-05-15)Som en del av Barents Sea Drill Cuttings Research Initiative (BARCUT) fokuserte denne masteroppgaven på å avdekke om bakteriesammensetningen i sedimentet rundt en borebrønn påvirkes av boreavfallsdeponeringer. Under tokt med M/S «Njord Viking» 30. november – 3. desember 2013 ble det hentet opp kjerneprøver ved en letebrønn i Brønnlokaliteten. Dette ble gjort samtidig med at Akvaplan-niva gjennomførte ... -
From Lab to Industry - Enzymes from Undifferentiated Marine Rest Raw Material
(Master thesis; Mastergradsoppgave, 2014-05-15)The aim of this project was to optimize the extraction of esterase enzymes from undifferentiated herring rest raw material. This was conducted by comparing, in laboratory scale, the outcome of using homogenised and non-homogenised raw material, and by extracting with buffers of different pH. Further on, clarification experiments of flocculation were performed on the optimal extract. The focus was ... -
SAV Ag CpG/polyI:C formulated vaccination potentiate protective immune responses in Atlantic salmon - no additive or synergistic effect present when co-injecting a Rhabdovirus G-DNA vaccine
(Master thesis; Mastergradsoppgave, 2013-02-11)Vaccines of today rely on adjuvant efficacy to provide a link between innate and adaptive immunity. CpG oligodeoxynucleotides (ODN) and polyinosinic:polycytidylic acid (polyI:C) are both toll-like receptor (TLR) ligands signaling through a combination of pathogen recognition receptors (PRR) and in mammals CpG/polyI:C are known to induce T-helper 1 (Th1) and cytotoxic T-lymphocyte (CTL) responses. ...