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dc.contributor.authorIngebrigtsen, Richard Andre
dc.contributor.authorHansen, Espen
dc.contributor.authorAndersen, Jeanette Hammer
dc.contributor.authorEilertsen, Hans Christian
dc.date.accessioned2018-01-04T13:47:24Z
dc.date.available2018-01-04T13:47:24Z
dc.date.issued2017-11-20
dc.description.abstractMicroalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of northern Norway and Spitsbergen were collected, extracted and fractionated. Biomass samples from a strain of a mass-cultivated diatom Porosira glacialis were used as a reference for comparison to field samples. Screening for bioactivity was performed with 13 assays within four therapeutic areas: antibacterial, anticancer, antidiabetes and antioxidation. We analysed the metabolic profiles of the samples using high resolution - mass spectroscopy (HR-MS). Principal component analysis showed a marked difference in metabolite profiles between the field samples and the photobioreactor culture; furthermore, the number of active fractions and extent of bioactivity was different in the field compared to the photobioreactor samples. We found varying levels of bioactivity in all samples, indicating that complex marine field samples could be used to investigate bioactivities from otherwise inaccessible sources. Furthermore, we hypothesize that metabolic pathways that would otherwise been silent under controlled growth in monocultures, might have been activated in the field samples.en_US
dc.descriptionSource at <a href=https://doi.org/10.1038/s41598-017-15980-8> https://doi.org/10.1038/s41598-017-15980-8 </a>en_US
dc.identifier.citationIngebrigtsen RA, Hansen E, Andersen JH, Eilertsen HC. Field sampling marine plankton for biodiscovery. Scientific Reports. 2017;7en_US
dc.identifier.cristinIDFRIDAID 1523031
dc.identifier.doidoi:10.1038/s41598-017-15980-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/11911
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.journalScientific Reports
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en_US
dc.titleField sampling marine plankton for biodiscoveryen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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