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dc.contributor.authorKumari, Jaya
dc.contributor.authorFlaten, Gøril Eide
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorTveiten, Helge
dc.date.accessioned2018-03-26T11:33:47Z
dc.date.available2018-03-26T11:33:47Z
dc.date.issued2017-06-15
dc.description.abstractThe delivery of exogenous biomolecules into teleost eggs is currently mostly relying on the manual microinjection methods, which, due to their high costs and low throughput, are not economically feasible for large-scale aquaculture applications. The main objective of this study was to develop a convenient, simple immersion delivery model for unfertilized Atlantic salmon (Salmo salar L.) eggs using liposomes as a system for the delivery of selected molecules. We used a lipophilic marker, Rhodamine B isothiocyanate (RBITC) dye, to follow liposomal uptake, marker release and marker distribution within incubated egg. We investigated the influence of the incubation time, liposome surface charges and liposome sizes on the uptake into the eggs. After incubation with liposomes, the exposed eggs were fertilized and embryonic development was monitored until complete yolk sac absorption. The result showed that RBITC, was successfully delivered to the yolk of the incubated eggs. Moreover, during embryonic development, liposomal RBITC remained in the yolk sac until the yolk was completely absorbed. The findings demonstrated a novel approach for the delivery of exogenous molecules to unfertilized Atlantic salmon eggs, opening an avenue for large-scale aquaculture therapeutic applications.en_US
dc.descriptionPublished version available in <a href=http://dx.doi.org/10.1016/j.aquaculture.2017.06.019> Aquaculture. 2017;479:404-411. </a>en_US
dc.identifier.citationKumari, J., Flaten, G. E., Škalko-Basnet, N., Tveiten, H. (2017). Molecular transfer to Atlantic salmon ovulated eggs using liposomes. Aquaculture, 479:404-411.en_US
dc.identifier.cristinIDFRIDAID 1476627
dc.identifier.doi10.1016/j.aquaculture.2017.06.019
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttps://hdl.handle.net/10037/12445
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalAquaculture
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/221648/NORWAY/Sterile salmon by targeting factors involved in germ cell survival: novel vaccination strategies for sustainable fish farming/Sketch 40/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922en_US
dc.subjectDeliveryen_US
dc.subjectLiposomesen_US
dc.subjectRhodamine Ben_US
dc.subjectRhodamine B isothiocyanateen_US
dc.subjectEggen_US
dc.subjectAtlantic salmonen_US
dc.titleMolecular transfer to Atlantic salmon ovulated eggs using liposomesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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