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dc.contributor.authorFrid, Paula
dc.contributor.authorBaraniya, Divyashri
dc.contributor.authorHalbig, Josefine Mareile
dc.contributor.authorRypdal, Veronika Gjertsen
dc.contributor.authorSongstad, Nils Thomas
dc.contributor.authorRosén, Annika
dc.contributor.authorBerstad, Johanna Elisabeth Rykke
dc.contributor.authorFlatø, Berit
dc.contributor.authorAlakwaa, Fadhl
dc.contributor.authorGil, Elisabeth Grut
dc.contributor.authorCetrelli, Lena Elisabet
dc.contributor.authorChen, Tsute
dc.contributor.authorAl-hebshi, Nezar Noor
dc.contributor.authorNordal, Ellen Berit
dc.contributor.authorAl-Haroni, Mohammed
dc.date.accessioned2020-12-09T11:36:31Z
dc.date.available2020-12-09T11:36:31Z
dc.date.issued2020-11-04
dc.description.abstract<i>Background</i>: The oral microbiota has been connected to the pathogenesis of rheumatoid arthritis through activation of mucosal immunity. The objective of this study was to characterize the salivary oral microbiome associated with juvenile idiopathic arthritis (JIA), and correlate it with the disease activity including gingival inflammation.<p> <i>Methods</i>: Fifty-nine patients with JIA (mean age, 12.6 ± 2.7 years) and 34 healthy controls (HC; mean age 12.3 ± 3.0 years) were consecutively recruited in this Norwegian cross-sectional study. Information about demographics, disease activity, medication history, frequency of tooth brushing and a modified version of the gingival bleeding index (GBI) and the simplified oral hygiene index (OHI-S) was obtained. Microbiome profiling of saliva samples was performed by sequencing of the V1-V3 region of the 16S rRNA gene, coupled with a species-level taxonomy assignment algorithm; QIIME, LEfSe and R-package for Spearman correlation matrix were used for downstream analysis.<p> <p><i>Results</i>: There were no significant differences between JIA and HC in alpha- and beta-diversity. However, differential abundance analysis revealed several taxa to be associated with JIA: <i>TM7-G1</i>, <i>Solobacterium</i> and <i>Mogibacterium</i> at the genus level; and <i>Leptotrichia</i> oral taxon 417, <i>TM7-G1</i> oral taxon 352 and <i>Capnocytophaga</i> oral taxon 864 among others, at the species level. <i>Haemophilus</i> species, <i>Leptotrichia</i> oral taxon 223, and <i>Bacillus</i> subtilis, were associated with healthy controls. <i>Gemella morbillorum</i>, <i>Leptotrichia</i> sp. oral taxon 498 and <i>Alloprevotella</i> oral taxon 914 correlated positively with the composite juvenile arthritis 10-joint disease activity score (JADAS10), while <i>Campylobacter</i> oral taxon 44 among others, correlated with the number of active joints. Of all microbial markers identified, only <i>Bacillus subtilis</i> and <i>Campylobacter</i> oral taxon 44 maintained false discovery rate (FDR) < 0.1.<p> <p><i>Conclusions</i>: In this exploratory study of salivary oral microbiome we found similar alpha- and beta-diversity among children with JIA and healthy. Several taxa associated with chronic inflammation were found to be associated with JIA and disease activity, which warrants further investigation.en_US
dc.identifier.citationFrid, Baraniya, Halbig, Rypdal, Songstad, Rosén, Berstad, Flatø, Alakwaa, Gil, Cetrelli, Chen, Al-hebshi, Nordal, Al-Haroni. Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study. Frontiers in Cellular and Infection Microbiology. 2020;10:1-13en_US
dc.identifier.cristinIDFRIDAID 1855791
dc.identifier.doi10.3389/fcimb.2020.602239
dc.identifier.issn2235-2988
dc.identifier.urihttps://hdl.handle.net/10037/20041
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Cellular and Infection Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Clinical dentistry disciplines: 830en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk odontologiske fag: 830en_US
dc.titleSalivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Studyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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