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dc.contributor.authorKloos, Julia Maria
dc.contributor.authorJohnsen, Pål Jarle
dc.contributor.authorHarms, Klaus
dc.date.accessioned2021-03-25T11:54:31Z
dc.date.available2021-03-25T11:54:31Z
dc.date.issued2020-12-03
dc.description.abstractTransposons are genetic elements that change their intracellular genomic position by transposition and are spread horizontally between bacteria when located on plasmids. It was recently discovered that transposition from fully heterologous DNA also occurs in the course of natural transformation. Here, we characterize the molecular details and constraints of this process using the replicative transposon Tn<i>1</i> and the naturally competent bacterium <i>Acinetobacter baylyi</i> . We find that chromosomal insertion of Tn<i>1</i> by transposition occurs at low but detectable frequencies and preferably around the <i>A. baylyi</i> terminus of replication. We show that Tn<i>1</i> transposition is facilitated by transient expression of the transposase and resolvase encoded by the donor DNA. RecA protein is essential for the formation of a circular, double-stranded cytoplasmic intermediate from incoming donor DNA, and RecO is beneficial but not essential in this process. Absence of the recipient RecBCD nuclease stabilizes the double-stranded intermediate. Based on these results, we suggest a mechanistic model for transposition during natural transformation.en_US
dc.identifier.citationKloos JM, Johnsen Pj, Harms K. Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi. Microbiology. 2020en_US
dc.identifier.cristinIDFRIDAID 1899981
dc.identifier.doi10.1099/mic.0.001003
dc.identifier.issn1350-0872
dc.identifier.issn1465-2080
dc.identifier.urihttps://hdl.handle.net/10037/20734
dc.language.isoengen_US
dc.publisherMicrobiology Societyen_US
dc.relation.ispartofKloos, J.M. (2021). Horizontal transfer, selection and maintenance of antibiotic resistance determinants. (Doctoral thesis). <a href=https://hdl.handle.net/10037/21191>https://hdl.handle.net/10037/21191</a>
dc.relation.journalMicrobiology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMED2/275672/Norway/Molecular and evolutionary characterization of short-patch double illegitimate recombination, a recently discovered mutation mechanism//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.titleTn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyien_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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