The use of traditional and unconventional culturing methods for the discovery of antimicrobial compounds derived from marine microorganisms

Abstract

The main aim is to evaluate the content of marine biofilms and different marine bacteria for antimicrobial potential. Hypothesis: antimicrobial compounds can be produced in the presence of other bacterial species; in the context of Winogradsky columns that simulate a naturalistic environment, and in the proximity to other species in the case of marine bacterial isolates. For this main aim and hypothesis, the following subgoals are defined: 1. Document any antimicrobial production by marine bacteria from different marine sources, using a co-culture approach. 2. Document the bacterial content of biofilm in Winogradsky columns (WC) from the seashore, using different techniques. 3. Evaluate the antibacterial production in the bacterial content from Winogradsky biofilms by performing diffusing assays, MIC assays in combinations with chromatographic methods.