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dc.contributor.authorAli, Jawad
dc.contributor.authorJoshi, Mukund
dc.contributor.authorAhmadi, Asal
dc.contributor.authorStrætkvern, Knut Olav
dc.contributor.authorAhmad, Rafi
dc.date.accessioned2023-08-14T06:36:55Z
dc.date.available2023-08-14T06:36:55Z
dc.date.issued2023-02-03
dc.description.abstractBackground: The rapid diagnostics of pathogens is essential to prescribe appropriate and early antibiotic therapy. The current methods for pathogen detection require the bacteria to grow in a culture medium, which is time-consuming. This increases the mortality rate and the global burden of antimicrobial resistance. Culture-free detection methods are still under development and are not used in the clinical routine. Therefore decreasing the culture time for accurate detection of infection and resistance is vital for diagnosis. <p><p>Methods: In this study, we wanted to investigate easy-to-implement factors (in a minimal laboratory set-up), including inoculum size, incubation temperature, and additional supplementation (e.g., vitamin B12 and trace metals), that can significantly reduce the lag time (t<sub>lag</lag>). These factors were arranged in simple two-level factorial designs using Gram-positive (Escherichia coli and Pseudomonas aeruginosa) and Gram-negative (Staphylococcus aureus and Bacillus subtilis) bacteria, including clinical isolates with known antimicrobial resistance profiles. Blood samples spiked with a clinical isolate of E. coli CCUG17620 were also tested to see the effect of elevated incubation temperature on bacterial growth in blood cultures. <p>Results: We observed that increased incubation temperature (42°C) along with vitamin B12 supplementation significantly reduced the t<sub>lag</sub> (10 – 115 minutes or 4% - 49%) in pure clinical isolates and blood samples spiked with E. coli CCUG17620. In the case of the blood sample, PCR results also detected bacterial DNA after only 3h of incubation and at three times the CFU/mL. <p>Conclusions: Enrichment of bacterial culture media with growth supplements such as vitamin B12 and increased incubation temperature can be a cheap and rapid method for the early detection of pathogens. This is a proof-of-concept study restricted to a few bacterial strains and growth conditions. In the future, the effect of other growth conditions and difficult-to-culture bacteria should be explored to shorten the lag phase.en_US
dc.identifier.citationAli j, Joshi M, Ahmadi A, Strætkvern KO, Ahmad R. Increased growth temperature and vitamin B12 supplementation reduces the lag time for rapid pathogen identification in BHI agar and blood cultures. F1000 Research. 2023;12(131)en_US
dc.identifier.cristinIDFRIDAID 2118443
dc.identifier.doi10.12688/f1000research.129668.2
dc.identifier.issn2046-1402
dc.identifier.urihttps://hdl.handle.net/10037/29886
dc.language.isoengen_US
dc.publisherF1000Researchen_US
dc.relation.journalF1000 Research
dc.relation.urihttps://f1000research.com/articles/12-131/v1
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleIncreased growth temperature and vitamin B12 supplementation reduces the lag time for rapid pathogen identification in BHI agar and blood culturesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)