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dc.contributor.authorTveit, Alexander
dc.contributor.authorSöllinger, Andrea
dc.contributor.authorRainer, Edda Marie
dc.contributor.authorDidriksen, Alena
dc.contributor.authorHestnes, Anne Grethe
dc.contributor.authorMotleleng, Liabo
dc.contributor.authorHellinger, Hans-Jörg
dc.contributor.authorRattei, Thomas
dc.contributor.authorSvenning, Mette Marianne
dc.date.accessioned2023-08-15T07:17:51Z
dc.date.available2023-08-15T07:17:51Z
dc.date.issued2023-01-18
dc.description.abstractMethanotrophs oxidize most of the methane (CH<sub>4</sub>) produced in natural and anthropogenic ecosystems. Often living close to soil surfaces, these microorganisms must frequently adjust to temperature change. While many environmental studies have addressed temperature effects on CH<sub>4</sub> oxidation and methanotrophic communities, there is little knowledge about the physiological adjustments that underlie these effects. We have studied thermal acclimation in Methylobacter, a widespread, abundant, and environmentally important methanotrophic genus. Comparisons of growth and CH<sub>4</sub> oxidation kinetics at different temperatures in three members of the genus demonstrate that temperature has a strong influence on how much CH<sub>4</sub> is consumed to support growth at different CH<sub>4</sub> concentrations. However, the temperature effect varies considerably between species, suggesting that how a methanotrophic community is composed influences the temperature effect on CH<sub>4</sub> uptake. To understand thermal acclimation mechanisms widely we carried out a transcriptomics experiment with Methylobacter tundripaludum SV96<sup>T</sup> . We observed, at different temperatures, how varying abundances of transcripts for glycogen and protein biosynthesis relate to cellular glycogen and ribosome concentrations. Our data also demonstrated transcriptional adjustment of CH<sub>4</sub> oxidation, oxidative phosphorylation, membrane fatty acid saturation, cell wall composition, and exopolysaccharides between temperatures. In addition, we observed differences in M. tundripaludum SV96<sup>T</sup> cell sizes at different temperatures. We conclude that thermal acclimation in Methylobacter results from transcriptional adjustment of central metabolism, protein biosynthesis, cell walls and storage. Acclimation leads to large shifts in CH<sub>4</sub> consumption and growth efficiency, but with major differences between species. Thus, our study demonstrates that physiological adjustments to temperature change can substantially influence environmental CH<sub>4</sub> uptake rates and that consideration of methanotroph physiology might be vital for accurate predictions of warming effects on CH<sub>4</sub> emissions.en_US
dc.identifier.citationTveit, Söllinger, Rainer, Didriksen, Hestnes, Motleleng, Hellinger, Rattei, Svenning. Thermal acclimation of methanotrophs from the genus Methylobacter. The ISME Journal. 2023;17(4):502-513en_US
dc.identifier.cristinIDFRIDAID 2140783
dc.identifier.doi10.1038/s41396-023-01363-7
dc.identifier.issn1751-7362
dc.identifier.issn1751-7370
dc.identifier.urihttps://hdl.handle.net/10037/29921
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalThe ISME Journal
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleThermal acclimation of methanotrophs from the genus Methylobacteren_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)