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dc.contributor.authorKuzmiszyn, Adrina Kalasho
dc.contributor.authorSelli, Anders Lund
dc.contributor.authorFuruholmen, Markus
dc.contributor.authorSmaglyukova, Natalia
dc.contributor.authorKondratiev, Timofei
dc.contributor.authorFuskevåg, Ole Martin
dc.contributor.authorSager, Georg
dc.contributor.authorDietrichs, Erik Sveberg
dc.date.accessioned2023-08-29T07:31:17Z
dc.date.available2023-08-29T07:31:17Z
dc.date.issued2023-01-14
dc.description.abstractRewarming from accidental hypothermia could be complicated by acute cardiac dysfunction but providing supportive pharmacotherapy at low core temperatures is challenging. Several pharmacological strategies aim to improve cardiovascular function by increasing cAMP in cardiomyocytes as well as cAMP and cGMP levels in vascular smooth muscle, but it is not clear what effects temperature has on cellular elimination of cAMP and cGMP. We therefore studied the effects of differential temperatures from normothermia to deep hypothermia (37 ◦C–20 ◦C) on cAMP levels in embryonic H9c2 cardiac cells and elimination of cAMP and cGMP by PDEenzymes and ABC-transporter proteins. Our experiments showed significant elevation of intracellular cAMP in H9c2-cells at 30 ◦C but not 20 ◦C. Elimination of both cAMP and cGMP through ABC transport-proteins and PDEenzymes showed a temperature dependent reduction. Accordingly, the increased cardiomyocyte cAMP-levels during moderate hypothermia appears an effect of preserved production and reduced elimination at 30 ◦C. This correlates with earlier in vivo findings of a positive inotropic effect of moderate hypothermia.en_US
dc.identifier.citationKuzmiszyn, AK, Selli AL, Furuholmen M, Smaglyukova NN, Kondratiev T, Fuskevåg O, Sager gs, Dietrichs ES. Moderate but not severe hypothermia increases intracellular cyclic AMP through preserved production and reduced elimination. Cryobiology. 2023en_US
dc.identifier.cristinIDFRIDAID 2107686
dc.identifier.doi10.1016/j.cryobiol.2023.01.005
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.urihttps://hdl.handle.net/10037/30490
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofKuzmiszyn, A.K. (2023). Temperature-dependent effects of phosphodiesterase inhibitors for cardiovascular support in hypothermic patients - Effects on cellular elimination of cAMP and cGMP. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31584>https://hdl.handle.net/10037/31584</a>.
dc.relation.journalCryobiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleModerate but not severe hypothermia increases intracellular cyclic AMP through preserved production and reduced eliminationen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)