Show simple item record

dc.contributor.authorMcClusky, Leon Mendel
dc.date.accessioned2023-09-11T11:15:23Z
dc.date.available2023-09-11T11:15:23Z
dc.date.issued2020-10-26
dc.description.abstractThis study was a single time-point mapping of various immunostaining patterns revealed with the proliferating cell nuclear antigen (PCNA) PC10 antibody in spermatogonia at the immature pole of the testis of the Blue shark (Prionace glauca). Scattered in the stroma of the germinal ridge that demarcates the immature pole's outer boundary were nests of variously immunoreactive A-spermatogonia, each flanked by a fusiform cell. Spermatocysts were assembled from niche-derived stromal cells, displaced A-progenitors, and their progeny, which showed one of two main immunostaining patterns (i.e., an uneven light brown/globular and homogeneous dark [hod] brown appearance). The testes of wild-caught Prionace showed two conditions, namely, extensive multinucleate cell death (MNC) near the mitosis–meiosis transition or an early recovery phase from the latter showing vacuolated areas. Both the proportion of cysts with immature Bhod-spermatogonia and the frequency of mitotic figures in such cysts in the early recovery testis condition were significantly higher than the comparable parameters in MNC testis condition. Moreover, the post-MNC recovery phase revealed a decrease in the proportion of immature cysts with uneven light brown/globular-like spermatogonia. The protracted spread of a cell cycle signal in an anatomically discrete, syncytially connected spermatogonial clone manifests as different PCNA immunoreactivities.en_US
dc.descriptionThis is the peer reviewed version of the following article: McClusky L. Simple, once‐off mapping of various, recurrent immunostaining patterns of proliferating cell nuclear antigen in spermatogonia at the immature pole of the testis of adult wild‐caught blue shark, Prionace glauca: Correlations with changes in testicular status. Molecular Reproduction and Development. 2020;87(11):1111-1123, which has been published in final form at https://doi.org/10.1002/mrd.23429. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.en_US
dc.identifier.citationMcClusky L. Simple, once‐off mapping of various, recurrent immunostaining patterns of proliferating cell nuclear antigen in spermatogonia at the immature pole of the testis of adult wild‐caught blue shark, Prionace glauca: Correlations with changes in testicular status. Molecular Reproduction and Development. 2020;87(11):1111-1123en_US
dc.identifier.cristinIDFRIDAID 1849715
dc.identifier.doi10.1002/mrd.23429
dc.identifier.issn1040-452X
dc.identifier.issn1098-2795
dc.identifier.urihttps://hdl.handle.net/10037/30911
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalMolecular Reproduction and Development
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.titleSimple, once‐off mapping of various, recurrent immunostaining patterns of proliferating cell nuclear antigen in spermatogonia at the immature pole of the testis of adult wild‐caught blue shark, Prionace glauca: Correlations with changes in testicular statusen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record