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dc.contributor.authorKnudsen, Steen Wilhelm
dc.contributor.authorHesselsøe, Martin
dc.contributor.authorRytter, Maria
dc.contributor.authorLillemark, Marie Rathcke
dc.contributor.authorTøttrup, Anders P.
dc.contributor.authorRahbek, Carsten
dc.contributor.authorSheard, Julie Koch
dc.contributor.authorThomsen, Philip Francis
dc.contributor.authorAgersnap, Sune
dc.contributor.authorMortensen, Peter B.
dc.contributor.authorMøller, Peter Rask
dc.date.accessioned2023-10-16T06:24:46Z
dc.date.available2023-10-16T06:24:46Z
dc.date.issued2023-08-25
dc.description.abstractMany species of amphibians in Northern Europe are threatened and the local distributions are rarely described in detail. Application of modern molecular methods provides an important supplementary tool for monitoring the distribution and diversity of amphibians. For this purpose, we designed, tested, validated, and optimized 14 species-specific assays on genomic DNA extracted from tissue samples to use for quantitative polymerase chain reaction (qPCR) setups targeting mitochondrial DNA from amphibians in freshwater samples. The tests confirmed species specificity for all assays. Considering a systematic definition of the limit of detection for each of the assays, the presented qPCR assays are unlikely to return false positive detection from any co-occurring species in northern Europe. For field validation, the qPCR assays were applied in a large-scale nationwide citizen science project in which sampling and qPCR analysis was carried out by high school students. Data from the citizen science project returned the expected results when compared to the known regional distribution of the target species and confirmed the presence of nine out of 14 Danish species of amphibians in the collected freshwater samples. Four out of 2550 qPCR test sets carried out by the high school students required a professional reanalysis in multiple replicates due to initial unexpected results. This emphasizes that efforts from citizen science may generate large amounts of valuable data, as long as the results are carefully scrutinized by experts.en_US
dc.identifier.citationKnudsen SW, Hesselsøe M, Rytter M, Lillemark MR, Tøttrup AP, Rahbek C, Sheard JK, Thomsen PF, Agersnap S, Mortensen PB, Møller PDR. Detection of environmental DNA from amphibians in Northern Europe applied in citizen science. Environmental DNA. 2023en_US
dc.identifier.cristinIDFRIDAID 2184069
dc.identifier.doi10.1002/edn3.462
dc.identifier.issn2637-4943
dc.identifier.urihttps://hdl.handle.net/10037/31561
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEnvironmental DNA
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleDetection of environmental DNA from amphibians in Northern Europe applied in citizen scienceen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)