Effect of 11-ketotestosterone on sperm production by male spotted wolffish (Anarhichas minor)
Permanent lenke
https://hdl.handle.net/10037/329Dato
2005-05-30Type
Master thesisMastergradsoppgave
Forfatter
Kugathaj, SubramaniamSammendrag
Three experiments were performed during spring 2004, to develop a method to elevate
the blood plasma level of 11-ketotestosterone (11-KT) in male brood stock of spotted
wolffish Anarhichas minor, and to study the effects of 11-KT on sperm production.
Intraperitoneal implantation of silastic capsules containing 11-KT - coconut butter
mixture and 11-KT - sesame oil mixture and intramuscular injection of 11-KT –
propylene glycol mixture were tested. Blood plasma 11-KT levels were quantified by
radioimmunoassay. Low and medium doses (c. 0.09 mg/kg and c. 0.18 mg/kg) of 11-
KT-coconut butter mixture administered in silastic capsules did not elevate the plasma
11-KT levels. High dose (c. 0.37 mg/kg) of 11-KT – coconut butter mixture gave an
increase (6.48 ± 1.89ng/ml) of about 3 times pretreatment level (1.83 ± 0.36 ng/ml) for
one week. 11-KT- sesame oil mixture (1 mg/kg dose) elevated the plasma 11-KT levels
(22.58 ± 4.69 ng/ml) compared to the controls (14.98 ± 3.45 ng/ml) for one week.
Silastic capsule implants did not maintain the elevation more than one week
irrespective of dose or vehicle (coconut butter, sesame oil). Intramuscular injection of
11-KT dissolved in propylene glycol (1mg/kg) elevated plasma 11-KT (99.16 ± 56.04
ng/ml) to about 12 times the level of the controls (7.44 ± 2.04 ng/ml), and was
identified as a more effective method than silastic capsule implantation. Sperm
production was studied by examining milt volume, spermatocrit, milt pH and motility
of sperm. 11-KT treatment with intramuscular injection significantly increased milt
volume. Mean milt volumes in treatment and control groups were 14.26 ± 4.2 ml and
4.73 ± 2.8 ml respectively. 11-KT treatment had no significant effects on spermatocrit,
which was very low (2 -4 %) in both treatment and control fish. Total sperm cell
volume in milt was significantly high in treatment group. There were no significant
differences in milt pH and sperm motility between 11-KT treatment and control groups.
The results indicate that 11-KT induces the spermatogenesis but does not influence the
motility of sperm.
Forlag
Universitetet i TromsøUniversity of Tromsø
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