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dc.contributor.authorFlo, Snorre
dc.contributor.authorVader, Anna
dc.contributor.authorPræbel, Kim
dc.date.accessioned2024-09-11T07:49:35Z
dc.date.available2024-09-11T07:49:35Z
dc.date.issued2024-05-06
dc.description.abstractPrey metabarcoding has become a popular tool in molecular ecology for resolving trophic interactions at high resolution, from various sample types and animals. To date, most predator–prey studies of small-sized animals (<1 mm) have met the problem of overabundant predator DNA in dietary samples by adding blocking primers/ peptide nucleic acids. These primers aim to limit the PCR amplification and detection of the predator DNA but may introduce bias to the prey composition identified by interacting with sequences that are similar to those of the predator. Here we demonstrate the use of an alternative method to explore the prey of small marine copepods using whole-body DNA extracts and deep, brute force metabarcoding of an 18S rDNA fragment. After processing and curating raw data from two sequencing runs of varying depths (0.4 and 5.4 billion raw reads), we isolated 1.3 and 52.2 million prey reads, with average depths of ~15,900 and ~120,000 prey reads per copepod individual, respectively. While data from both sequencing runs were sufficient to distinguish dietary compositions from disparate seasons, locations, and copepod species, greater sequencing depth led to better separation of clusters. As computation and sequencing are becoming ever more powerful and affordable, we expect the brute force approach to become a general standard for prey metabarcoding, as it offers a simple and affordable solution to consumers that is impractical to dissect or unknown to science.en_US
dc.identifier.citationFlo, Vader, Præbel. Brute force prey metabarcoding to explore the diets of small invertebrates. Ecology and Evolution. 2024;14(5)en_US
dc.identifier.cristinIDFRIDAID 2266710
dc.identifier.doi10.1002/ece3.11369
dc.identifier.issn2045-7758
dc.identifier.urihttps://hdl.handle.net/10037/34678
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEcology and Evolution
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleBrute force prey metabarcoding to explore the diets of small invertebratesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)