Parasitic dodder expresses an arsenal of secreted cellulases with multi-substrate specificity during host invasion
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https://hdl.handle.net/10037/35105Dato
2024-04-17Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Edema, Hilary; Bawin, Thomas Georges A; Olsen, Stian; Krause, Kirsten; Karppinen, Katja HanneleSammendrag
Cuscuta campestris is a common and problematic parasitic plant which relies on haustoria to connect to and
siphon nutrients from host plants. Glycoside hydrolase family 9 (GH9) cellulases (EC 3.2.1.4) play critical roles in
plant cell wall biosynthesis and disassembly, but their roles during Cuscuta host invasion remains underexplored.
In this study, we identified 22 full-length GH9 cellulase genes in C. campestris genome, which encoded fifteen
secreted and seven membrane-anchored cellulases that showed distinct phylogenetic relationships. Expression
profiles suggested that some of the genes are involved in biosynthesis and remodeling of the parasite’s cell wall
during haustoriogenesis, while other genes encoding secreted B- and C-type cellulases are tentatively associated
with degrading host cell walls during invasion. Transcriptomic data in a host-free system and in the presence of
susceptible or partially resistant tomato hosts, showed for especially GH9B7, GH9B11 and GH9B12 a shift in
expression profiles in the presence of hosts, being more highly expressed during host attachment, indicating that
Cuscuta can tune cellulase expression in response to a host. Functional analyses of recombinant B- and C-type
cellulases showed endoglucanase activities over wide pH and temperature conditions, and activities towards
multiple cellulose and hemicellulose substrates. These findings improve our understanding of host cell wall
disassembly by Cuscuta, and cellulase activity towards broad substrate range potentially explain its wide host
range. This is the first study to provide a broad biochemical insight into Cuscuta GH9 cellulases, which based on
our study may have potential applications in industrial bioprocessing.
Forlag
ElsevierSitering
Edema, Bawin, Olsen, Krause, Karppinen. Parasitic dodder expresses an arsenal of secreted cellulases with multi-substrate specificity during host invasion. Plant physiology and biochemistry (Paris). 2024;210Metadata
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