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dc.contributor.authorLorentzen, Øyvind Myrvoll
dc.contributor.authorBleis, Christina
dc.contributor.authorAbel, Sören
dc.date.accessioned2024-11-04T09:15:52Z
dc.date.available2024-11-04T09:15:52Z
dc.date.issued2024-08-23
dc.description.abstractNext-generation sequencing methods have become essential for studying bacterial biology and pathogenesis, often depending on high-quality, closed genomes. In this study, we utilized a hybrid sequencing approach to assemble the genome of C6706, a widely used Vibrio cholerae model strain. We present a manually curated annotation of the genome, enhancing user accessibility by linking each coding sequence to its counterpart in N16961, the first sequenced V. cholerae isolate and a commonly used reference genome. Comparative genomic analysis between V. cholerae C6706 and N16961 uncovered multiple genetic differences in genes associated with key biological functions. To determine whether these genetic variations result in phenotypic differences, we compared several phenotypes relevant to V. cholerae pathogenicity like genetic stability, acid sensitivity, biofilm formation and motility. Notably, V. cholerae N16961 exhibited greater motility and reduced biofilm formation compared to V. cholerae C6706. These phenotypic differences appear to be mediated by variations in quorum sensing and cyclic di-GMP signalling pathways between the strains. This study provides valuable insights into the regulation of biofilm formation and motility in V. cholerae.en_US
dc.identifier.citationLorentzen, Bleis, Abel. A comparative genomic and phenotypic study of Vibrio cholerae model strains using hybrid sequencing. Microbiology (Reading). 2024;170(9)en_US
dc.identifier.cristinIDFRIDAID 2309922
dc.identifier.doi10.1099/mic.0.001502
dc.identifier.issn1350-0872
dc.identifier.issn1465-2080
dc.identifier.urihttps://hdl.handle.net/10037/35419
dc.language.isoengen_US
dc.publisherMicrobiology Societyen_US
dc.relation.journalMicrobiology (Reading)
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleA comparative genomic and phenotypic study of Vibrio cholerae model strains using hybrid sequencingen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)