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dc.contributor.authorChang, Chia Jung
dc.contributor.authorSun, Baojian
dc.contributor.authorRobertsen, Børre
dc.date.accessioned2016-03-17T09:59:36Z
dc.date.available2016-03-17T09:59:36Z
dc.date.issued2015-04-09
dc.description.abstractThere is a need for more efficient vaccines to combat viral diseases of Atlantic salmon and other farmed fish. DNA vaccines are highly effective against salmonid rhabdoviruses, but have shown less effect against other viruses. In the present work we have studied if type I IFNs might be used as adjuvants in fish DNA vaccines. For this purpose we chose a DNA vaccine model based on the hemagglutinin-esterase (HE) gene of infectious salmon anemia virus (ISAV) as antigen. Salmon presmolts were injected with a plasmid encoding HE alone or together with a plasmid encoding Atlantic salmon type I IFN (IFNa1, IFNb or IFNc). Sera were harvested after 7–10 weeks for measurements of antibody against ISAV and the fish were challenged with ISAV to measure protective effects of the vaccines. The results showed that all three IFN plasmids delivered together with HE plasmid potently enhanced protection of salmon against ISAV mediated mortality and stimulated an increase in IgM antibodies against the virus. In contrast, HE plasmid alone gave low antibody titers and a minor protection against ISAV. This demonstrates that type I IFNs stimulate adaptive immune responses in fish, which may be a benefit also in other fish DNA vaccines. Quantitative RT-PCR studies showed that the salmon IFNs caused an increased influx of B-cells and cytotoxic T-cells at the muscle injection site, which may in part explain the adjuvant effect of the IFNs.en_US
dc.descriptionPublished version also available at <a href=http://dx.doi.org/10.1016/j.vaccine.2015.03.093>http://dx.doi.org/10.1016/j.vaccine.2015.03.093</a>en_US
dc.identifier.citationVaccine 2015, 33(21):2442-2448en_US
dc.identifier.cristinIDFRIDAID 1256699
dc.identifier.doi10.1016/j.vaccine.2015.03.093
dc.identifier.issn0264-410X
dc.identifier.urihttps://hdl.handle.net/10037/9002
dc.identifier.urnURN:NBN:no-uit_munin_8566
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923en_US
dc.subjectIFNen_US
dc.subjectAdjuvanten_US
dc.subjectDNA vaccineen_US
dc.subjectInfectious salmon anemia virusen_US
dc.subjectAtlantic salmonen_US
dc.subjectAntibodyen_US
dc.subjectHemagglutinin-esteraseen_US
dc.titleAdjuvant activity of fish type I interferon shown in a virus DNA vaccination modelen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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