The Genetic Structures of an Extensively Drug Resistant (XDR) Klebsiella pneumoniae and its Plasmids
Permanent lenke
https://hdl.handle.net/10037/14683Dato
2019-01-04Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Li, Ling; Yu, Tao; Ma, Yanan; Yang, Zhongjun; Wang, Wenjia; Song, Xiaobo; Shen, Yu; Guo, Tingting; Kong, Jian; Wang, Mingyu; Xu, HaiSammendrag
Multi-, extensively-, and pan-drug resistant bacteria are a threat to our health today, because their wide resistance spectra make their infections difficult to cure. In this work, we isolated an extensively drug resistant (XDR) Klebsiella pneumoniae 2-1 strain from the stool sample of a patient diagnosed of colorectal cancer. K. pneumoniae 2-1 was found to be resistant to all the antibiotics tested except for cefepime, tigecycline, and ceftazidime-avibactam. By sequencing the complete genome of K. pneumoniae 2-1, we found it contains a chromosome of 5.23 Mb and two circular plasmids with the size of 246 and 90 kb. The larger plasmid, pKP21HI1 was found to be a new conjugation-defective plasmid belonging to incompatibility group HI1B and a new sequence type. Further comparative genomics analysis and antimicrobial resistance gene analysis showed that although a great deal of changes took place on the chromosome of K. pneumoniae 2-1 in comparison with the reference genome, the extensively drug resistance phenotype of K. pneumoniae 2-1 is primarily due to the two multidrug resistant plasmids it contains. This work explains the genetic and mechanistic basis of the extensive drug resistance of K. pneumoniae 2-1, and found that plasmids play key roles in the strong antibiotic resistance of bacteria.
Beskrivelse
Source at https://doi.org/10.3389/fcimb.2018.00446.
Forlag
Nature ResearchSitering
Li, L., Yu, T., Ma, Y., Yang, Z., Wang, W., Song, X.S., ... Xu, H. (2019). The Genetic Structures of an Extensively Drug Resistant (XDR) Klebsiella pneumoniae and Its Plasmids. Frontiers in Cellular and Infection Microbiology, 8, 446. https://doi.org/10.3389/fcimb.2018.00446Metadata
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