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dc.contributor.authorHadler-Olsen, Elin Synnøve
dc.contributor.authorWinberg, Jan-Olof
dc.date.accessioned2020-01-29T09:53:02Z
dc.date.available2020-01-29T09:53:02Z
dc.date.issued2019
dc.description.abstractTo explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present a protocol for real-time gelatin zymography that is very useful for the detection of gelatin-degrading proteases in tissue extracts. This method uses fluorescence-labeled gelatin and therefore we also present an easy, fast, and cheap method for labeling gelatin with 2-methoxy-2,4-diphenyl-3(<i>2H</i>)-furanone (MDPF).en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in <i>Methods in Molecular Biology, vol 1952</i>. The final authenticated version is available online at: <a href=https://doi.org/10.1007/978-1-4939-9133-4_16>https://doi.org/10.1007/978-1-4939-9133-4_16. </a>en_US
dc.identifier.citationHadler-Olsen E., Winberg JO. (2019) Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography. In: Vigetti D., Theocharis A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NYen_US
dc.identifier.cristinIDFRIDAID 1777218
dc.identifier.doihttps://doi.org/10.1007/978-1-4939-9133-4_16
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://hdl.handle.net/10037/17256
dc.language.isoengen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofseriesMethods in Molecular Biology, vol 1952
dc.relation.journalMethods in molecular biology
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© Springer Science+Business Media, LLC, part of Springer Nature 2019en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleMethod for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymographyen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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