Adding zooplankton to the OSMAC toolkit: Effect of grazing stress on the metabolic profile and bioactivity of a diatom
Permanent lenke
https://hdl.handle.net/10037/21272Dato
2021-02-03Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Osvik, Renate Døving; Ingebrigtsen, Richard Andre; Norrbin, Fredrika; Andersen, Jeanette Hammer; Eilertsen, Hans Christian; Hansen, Espen HolstSammendrag
“One strain many compounds” (OSMAC) based approaches have been widely used in the search for bioactive compounds. Introducing stress factors like nutrient limitation, UV-light or cocultivation with competing organisms has successfully been used in prokaryote cultivation. It is known that diatom physiology is affected by changed cultivation conditions such as temperature, nutrient concentration and light conditions. Cocultivation, though, is less explored. Hence, we wanted to investigate whether grazing pressure can affect the metabolome of the marine diatom Porosira glacialis, and if the stress reaction could be detected as changes in bioactivity. P. glacialis cultures were mass cultivated in large volume bioreactor (6000 L), first as a monoculture and then as a coculture with live zooplankton. Extracts of the diatom biomass were screened in a selection of bioactivity assays: inhibition of biofilm formation, antibacterial and cell viability assay on human cells. Bioactivity was found in all bioassays performed. The viability assay towards normal lung fibroblasts revealed that P. glacialis had higher bioactivity when cocultivated with zooplankton than in monoculture. Cocultivation with diatoms had no noticeable effect on the activity against biofilm formation or bacterial growth. The metabolic profiles were analyzed showing the differences in diatom metabolomes between the two culture conditions. The experiment demonstrates that grazing stress affects the biochemistry of P. glacialis and thus represents a potential tool in the OSMAC toolkit.
Er en del av
Osvik, R.D. (2021). Bioprospecting of marine phytoplankton from large scale cultivation - Effect of culture conditions on bioactivity and biochemistry of the diatom Porosira glacialis. (Doctoral thesis). https://hdl.handle.net/10037/21273Forlag
MDPISitering
Osvik RD, Ingebrigtsen RA, Norrbin MF, Andersen JH, Eilertsen HC, Hansen E. H.. Adding zooplankton to the OSMAC toolkit: Effect of grazing stress on the metabolic profile and bioactivity of a diatom. Marine Drugs. 2021;19Metadata
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