Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products
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https://hdl.handle.net/10037/23151Dato
2021-11-01Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Schneider, Yannik Karl-Heinz; Andersen, Jeanette Hammer; Hansen, Espen; Jørgensen, Solveig M.Sammendrag
A key step in the process of isolating microbial natural products is the preparation of an
extract from a culture. This step determines which molecules will be available for detection in the
subsequent chemical and biological analysis of a biodiscovery pipeline. In the present study we
wanted to document potential differences in performance between liquid–liquid extraction using
ethyl acetate and liquid–solid extraction using a poly‐benzyl‐resin. For the comparison of the two
extraction protocols, we spiked a culture of Flavobacterium sp. with a diverse selection of natural
products of microbial and plant origin to investigate whether the methods were comparable with
respect to selectivity. We also investigated the efficiency of the two extraction methods quantita‐
tively, using water spiked with a selection of natural products, and studied the quantitative effect
of different pH levels of the aqueous solutions on the extraction yields of the two methods. The
same compounds were extracted by the two methods, but the solid‐phase extract contained more
media components compared with the liquid‐phase extract. Quantitatively, the two extraction
methods varied in their recovery rates. We conclude that practical aspects could be more important
when selecting one of the extraction protocols, as their efficiencies in extracting specific compounds
were quite similar.
Sitering
Schneider, Jørgensen S, Andersen, Hansen. Qualitative and Quantitative Comparison of Liquid–Liquid Phase Extraction Using Ethyl Acetate and Liquid–Solid Phase Extraction Using Poly-Benzyl-Resin for Natural Products. Applied Sciences. 2021;11Metadata
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