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dc.contributor.authorLarsen, Anett Kristin
dc.contributor.authorSimon-Santamaria, Jaione
dc.contributor.authorElvevold, Kjetil
dc.contributor.authorEriczon, Bo Gøran
dc.contributor.authorMortensen, Kim Erlend
dc.contributor.authorMcCourt, Peter Anthony
dc.contributor.authorSmedsrød, Bård
dc.contributor.authorSørensen, Karen Kristine
dc.date.accessioned2022-02-21T10:13:57Z
dc.date.available2022-02-21T10:13:57Z
dc.date.issued2021-12-13
dc.description.abstractAutofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of exogenous fluorophores, especially when labelling of live cells where the quencher is not compatible.en_US
dc.identifier.citationLarsen AK, Simon-Santamaria J, Elvevold K, Ericzon, Mortensen KE, McCourt PAG, Smedsrød b, Sørensen KK. Autofluorescence in freshly isolated adult human liver sinusoidal cells. European journal of histochemistry. 2021;65(4)en_US
dc.identifier.cristinIDFRIDAID 1973961
dc.identifier.doi10.4081/ejh.2021.3337
dc.identifier.issn1121-760X
dc.identifier.issn2038-8306
dc.identifier.urihttps://hdl.handle.net/10037/24096
dc.language.isoengen_US
dc.publisherPAGEpressen_US
dc.relation.journalEuropean journal of histochemistry
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleAutofluorescence in freshly isolated adult human liver sinusoidal cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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