Show simple item record

dc.contributor.authorZambalde, Erika Pereira
dc.contributor.authorBayraktar, Recep
dc.contributor.authorJucoski, Tayana Schultz
dc.contributor.authorIvan, Cristina
dc.contributor.authorRodrigues, Ana Carolina de Mello Alves
dc.contributor.authorMathias, Carolina
dc.contributor.authorKnutsen, Erik
dc.contributor.authorSilveira de Lima, Rubens
dc.contributor.authorGradia, Daniela Fiori
dc.contributor.authorFonseca, Enilze Maria de Souza
dc.contributor.authorHannash, Samir
dc.contributor.authorCalin, George A.
dc.contributor.authorOliveira, Jaqueline Carvalhode
dc.date.accessioned2022-03-17T14:03:30Z
dc.date.available2022-03-17T14:03:30Z
dc.date.issued2021-08-13
dc.description.abstractThe human genome contains 481 ultraconserved regions (UCRs), which are genomic stretches of over 200 base pairs conserved among human, rat, and mouse. The majority of these regions are transcriptionally active (T-UCRs), and several have been found to be differentially expressed in tumours. Some T-UCRs have been functionally characterized, but of those few have been associated to breast cancer (BC). Using TCGA data, we found 302 T-UCRs related to clinical features in BC: 43% were associated with molecular subtypes, 36% with oestrogen-receptor positivity, 17% with HER2 expression, 12% with stage, and 10% with overall survival. The expression levels of 12 T-UCRs were further analysed in a cohort of 82 Brazilian BC patients using RT-qPCR. We found that uc.147 is high expressed in luminal A and B patients. For luminal A, a subtype usually associated with better prognosis, high uc.147 expression was associated with a poor prognosis and suggested as an independent prognostic factor. The lncRNA from uc.147 (lnc-uc.147) is located in the nucleus. Northern blotting results show that uc.147 is a 2,8 kb monoexonic trancript, and its sequence was confirmed by RACE. The silencing of uc.147 increases apoptosis, arrests cell cycle, and reduces cell viability and colony formation in BC cell lines. Additionally, we identifed 19 proteins that interact with lnc-uc.147 through mass spectrometry and demonstrated a high correlation of lnc-uc.147 with the neighbour gene expression and miR-18 and miR-190b. This is the first study to analyse the expression of all T-UCRs in BC and to functionally assess the lnc-uc.147.en_US
dc.descriptionThis is an Accepted Manuscript of an article published by Taylor & Francis in RNA Biology on 13. august 2021, available (including supplementary) material online: https://doi.org/10.1080/15476286.2021.1952757.en_US
dc.identifier.citationZambalde, Bayraktar R, Jucoski, Ivan C, Rodrigues ACdMA, Mathias, Knutsen E, Silveira de Lima, Gradia, Fonseca, Hannash, Calin GA, Oliveira. A novel lncRNA derived from an ultraconserved region: lnc- uc.147, a potential biomarker in luminal A breast cancer. RNA Biology. 2021;18(1):416-429en_US
dc.identifier.cristinIDFRIDAID 1975386
dc.identifier.doi10.1080/15476286.2021.1952757
dc.identifier.issn1547-6286
dc.identifier.issn1555-8584
dc.identifier.urihttps://hdl.handle.net/10037/24443
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relation.journalRNA Biology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleA novel lncRNA derived from an ultraconserved region: lnc- uc.147, a potential biomarker in luminal A breast canceren_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record