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dc.contributor.authorCui, Guanglin
dc.contributor.authorLi, Gui
dc.contributor.authorPang, Zhigang
dc.contributor.authorFlorholmen, Jon
dc.contributor.authorGoll, Rasmus
dc.date.accessioned2022-11-01T08:46:32Z
dc.date.available2022-11-01T08:46:32Z
dc.date.issued2022-06-07
dc.description.abstractBackground: Accumulative evidence suggests that the biological behavior of cancer stem-like cells (CSCs) is regulated by their surrounding niche, in which cytokines function as one of the main mediators for the interaction between CSCs and their microenvironment in the colorectal cancer (CRC).<p> <p>Methods: We characterized the presentation of CSCs and the interleukin (IL)− 8 network in the adenoma/CRC epithelium using quantitative real-time PCR (q-PCR), immunohistochemistry (IHC) and double immunofluorescence. In addition, the capacity of IL-1β to stimulate epithelial IL-8 production in colon cancer Caco-2 cells was examined in vitro and the IL-8 product was measured by enzyme-linked immunosorbent assay (ELISA). <p>Results: IHC observation showed increased expression of both CSCs and IL-8 in the adenoma and CRC epithelium, and q-PCR results revealed that increased expression of IL-1β transcript was strongly correlated with increased IL-8 transcript levels in both adenoma and CRC tissues. Double immunofluorescence images demonstrated the coexpression of the IL-8 receptors IL-8RA and IL-8RB with LGR5 labeled CSCs in CRC tissue sections. Consistently, in vitro experiments showed that coculture of Caco-2 cells with IL-1β at concentrations of 1, 5, 10 and 20 ng/ml resulted in a dose-dependent release of IL-8, which could be specifically inhibited by cotreatment with the IL-1β receptor antagonist. <p>Conclusions: These results demonstrate activation of the IL-8 network in the niche of CSCs from the precancerous adenoma stage to the CRC stage, which is potentially stimulated by IL-1β in CRC cells.en_US
dc.identifier.citationCui G, Li, Pang Z, Florholmen J, Goll r. The presentation and regulation of the IL-8 network in the epithelial cancer stem-like cell niche in patients with colorectal cancer. Biomedicine & Pharmacotherapy. 2022;152en_US
dc.identifier.cristinIDFRIDAID 2041946
dc.identifier.doi10.1016/j.biopha.2022.113252
dc.identifier.issn0753-3322
dc.identifier.issn1950-6007
dc.identifier.urihttps://hdl.handle.net/10037/27212
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalBiomedicine & Pharmacotherapy
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleThe presentation and regulation of the IL-8 network in the epithelial cancer stem-like cell niche in patients with colorectal canceren_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)