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dc.contributor.authorUntergasser, Andreas
dc.contributor.authorHellemans, Jan
dc.contributor.authorPfaffl, Michael W.
dc.contributor.authorRuijter, Jan M.
dc.contributor.authorvan den Hoff, Maurice J. B.
dc.contributor.authorDragomir, Mihnea P.
dc.contributor.authorAdamoski, Douglas
dc.contributor.authorDias, Sandra Martha Gomes
dc.contributor.authorReis, Rui Manuel
dc.contributor.authorFerracin, Manuela
dc.contributor.authorDias-Neto, Emmanuel
dc.contributor.authorMarsh, Ian
dc.contributor.authorKubista, Mikael
dc.contributor.authorFabbri, Muller
dc.contributor.authorGoel, Ajay
dc.contributor.authorSlabý, Ondřej
dc.contributor.authorKnutsen, Erik
dc.contributor.authorChen, Baoqing
dc.contributor.authorNegrini, Massimo
dc.contributor.authorMimori, Koshi
dc.contributor.authorPichler, Martin
dc.contributor.authorPapatriantafyllou, Maria
dc.contributor.authorAnfossi, Simone
dc.contributor.authorSchmittgen, Thomas D.
dc.contributor.authorHuggett, Jim
dc.contributor.authorBustin, Stephen
dc.contributor.authorVandesompele, Jo
dc.contributor.authorCalin, George A.
dc.date.accessioned2023-08-11T11:54:46Z
dc.date.available2023-08-11T11:54:46Z
dc.date.issued2023-03-14
dc.description.abstractAccuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.en_US
dc.identifier.citationUntergasser, Hellemans, Pfaffl, Ruijter, van den Hoff, Dragomir, Adamoski, Dias, Reis, Ferracin, Dias-Neto, Marsh, Kubista, Fabbri, Goel, Slabý, Knutsen, Chen, Negrini, Mimori, Pichler, Papatriantafyllou, Anfossi, Schmittgen, Huggett, Bustin, Vandesompele, Calin. Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action. Molecular Oncology. 2023;17(5):713-717en_US
dc.identifier.cristinIDFRIDAID 2159276
dc.identifier.doi10.1002/1878-0261.13418
dc.identifier.issn1574-7891
dc.identifier.issn1878-0261
dc.identifier.urihttps://hdl.handle.net/10037/29879
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalMolecular Oncology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleDisclosing quantitative RT-PCR raw data during manuscript submission: a call for actionen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)