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dc.contributor.authorPasserini, Sara
dc.contributor.authorPrezioso, Carla
dc.contributor.authorProta, Annalisa
dc.contributor.authorBabini, Giulia
dc.contributor.authorBargiacchi, Lavinia
dc.contributor.authorBartolini, Daniela
dc.contributor.authorMoens, Ugo
dc.contributor.authorAntonelli, Manila
dc.contributor.authorPietropaolo, Valeria
dc.date.accessioned2023-08-30T07:42:51Z
dc.date.available2023-08-30T07:42:51Z
dc.date.issued2023-04-25
dc.description.abstractDue to its peculiar histopathological findings, pleomorphic xanthoastrocytoma (PXA), a rare cerebral tumor of young adults with a slow growth and a good prognosis, resembles to the lytic phase of progressive multifocal leukoencephalopathy, a fatal neurodegenerative disease caused by JC polyomavirus (JCPyV). Therefore, the presence of JCPyV DNA was examined in an 11-year-old child with xanthoastrocytoma, WHO grade 3, by quantitative PCR (qPCR) and nested PCR (nPCR) using primers amplifying sequences encoding the N- and C-terminal region of large T antigen (LTAg), the non-coding control region (NCCR), and viral protein 1 (VP1) DNA. The expression of transcripts from LTAg and VP1 genes was also evaluated. In addition, viral microRNAs’ (miRNAs) expression was investigated. Cellular p53 was also searched at both DNA and RNA level. qPCR revealed the presence of JCPyV DNA with a mean value of 6.0× 104 gEq/mL. nPCR gave a positive result for the 5ʹ region of the LTAg gene and the NCCR, whereas 3ʹ end LTAg and VP1 DNA sequences were not amplifiable. Only LTAg transcripts of 5ʹ end were found whereas VP1 gene transcript was undetectable. Although in most cases, either Mad-1 or Mad-4 NCCRs have been identified in association with JCPyV-positive human brain neoplasms, the archetype NCCR structure was observed in the patient’s sample. Neither viral miRNA miR-J1-5p nor p53 DNA and RNA were detected. Although the expression of LTAg supports the possible role of JCPyV in PXA, further studies are warranted to better understand whether the genesis of xanthoastrocytoma could depend on the transformation capacity of LTAg by Rb sequestration.en_US
dc.identifier.citationPasserini, Prezioso, Prota, Babini, Bargiacchi, Bartolini, Moens, Antonelli, Pietropaolo. Detection of human neurotropic JCPyV DNA sequence in pediatric anaplastic xanthoastrocytoma. Journal of Neurovirology. 2023;29(2):232-236en_US
dc.identifier.cristinIDFRIDAID 2158403
dc.identifier.doi10.1007/s13365-023-01129-z
dc.identifier.issn1355-0284
dc.identifier.issn1538-2443
dc.identifier.urihttps://hdl.handle.net/10037/30524
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalJournal of Neurovirology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleDetection of human neurotropic JCPyV DNA sequence in pediatric anaplastic xanthoastrocytomaen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)