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dc.contributor.authorYang, Nannan
dc.contributor.authorHellevik, Turid
dc.contributor.authorBerzaghi, Rodrigo
dc.contributor.authorMartinez-Zubiaurre, Inigo
dc.date.accessioned2024-09-13T10:19:52Z
dc.date.available2024-09-13T10:19:52Z
dc.date.issued2024-03-15
dc.description.abstractBackground: Cancer-associated fibroblasts (CAFs) consist of heterogeneous connective tissue cells and are often constituting the most abundant cell type in the tumor stroma. Radiation effects on tumor stromal components like CAFs in the context of radiation treatment is not well-described.<p> <p>Aim: This study explores potential changes induced by ionizing radiation (IR) on platelet-derived growth factor (PDGF)/PDGFRs and transforming growth factor-beta (TGF-β)/TGFβRs signaling systems in CAFs. <p>Methods and Results: Experiments were carried out by employing primary cultures of human CAFs isolated from freshly resected non-small cell lung carcinoma tumor tissues. CAF cultures from nine donors were treated with one high (1 18 Gy) or three fractionated (3 6 Gy) radiation doses. Alterations in expression levels of TGFβRII and PDGFRα/β induced by IR were analyzed by western blots and flow cytometry. In the presence or absence of cognate ligands, receptor activation was studied in nonirradiated and irradiated CAFs. Radiation exposure did not exert changes in expression of PDGF or TGF-β receptors in CAFs. Additionally, IR alone was unable to trigger activation of either receptor. The radiation regimens tested did not affect PDGFRβ signaling in the presence of PDGF-BB. In contrast, signaling via pSmad2/3 and pSmad1/5/8 appeared to be down-regulated in irradiated CAFs after stimulation with TGF-β, as compared with controls. <p>Conclusion: Our data demonstrate that IR by itself is insufficient to induce measurable changes in PDGF or TGF-β receptor expression levels or to induce receptor activation in CAFs. However, in the presence of their respective ligands, exposure to radiation at certain doses appear to interfere with TGF-β receptor signaling.en_US
dc.identifier.citationYang, Hellevik, Berzaghi, Martinez-Zubiaurre. Radiation-induced effects on TGF-β and PDGF receptor signaling in cancer-associated fibroblasts. Cancer Reports. 2024;7(3)en_US
dc.identifier.cristinIDFRIDAID 2262515
dc.identifier.doi10.1002/cnr2.2018
dc.identifier.issn2573-8348
dc.identifier.urihttps://hdl.handle.net/10037/34705
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalCancer Reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleRadiation-induced effects on TGF-β and PDGF receptor signaling in cancer-associated fibroblastsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)