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norskMastergradsoppgaver i marin bioteknologi
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Selective Isolation of Actinobacteria from Marine Invertebrates: A Bio-Prospective Search for New Producer Strains
(Master thesis; Mastergradsoppgave, 2024-05-15)Actinobacteria are spore-forming Gram-positive bacteria, with high G-C content in their genome. Their ability to metabolize complex compounds helps them survive different environmental conditions and form diverse associations with different organisms. These characteristics make Actinobacteria good producers of natural products with useful bioactivity. However, Actinobacteria are slow growers and ... -
Secondary Metabolites from Marine Invertebrate Extracts
(Master thesis; Mastergradsoppgave, 2024-05-15)In drug discovery, natural organisms offer a rich source of bioactive compounds, with over one-third of drugs originating from natural sources. Bioprospecting is the field of systematically screening organisms to discover and isolate novel compounds for drug development or other applications. With the shifting trends of examining the biodiversity of the marine environment, have marine bioprospecting ... -
Exploring the Performance Space of a Genetic Toggle Switch across Host and RBS context
(Master thesis; Mastergradsoppgave, 2024-05-14)Synthetic biology applies engineering principles onto biological systems to rationally create novel devices with desired properties. The behavior of an engineered genetic circuit is influenced by a complex multitude of factors, including regulatory elements and the cellular environment it operates within. This thesis delves into the impact of ribosome binding site (RBS) configurations and host context ... -
Bacteriophages of the arctic bacterium Staphylococcus borealis - isolation, host range and anti-biofilm effect The evaluation of efficacy, host range and anti-biofilm capacities of bacteriophages infectious to nosocomial staphylococcal strains
(Master thesis; Mastergradsoppgave, 2024-05-13)As antibiotic resistance is on the rise in parallel with the stagnation of antibiotic discovery, the world urges for novel antibacterial strategies. Phage therapy has a century-long history of application in former Soviet countries in eastern Europe and has gained attention as a potential alternative to conventional antibiotics, with high specificity and low cytotoxicity. This study aimed at isolating ... -
Targeted gene editing of an infectious salmon anemia (ISA)-relevant gene in salmonids cells using the CRISPR/Cas9 Ribonucleoprotein complex
(Master thesis; Mastergradsoppgave, 2022-05-10)Background: The gene editing tool, CRISPR/Cas9, can potentially be applied in aquaculture to combat some of the problems of the farmed salmon industry including infectious diseases. The RNP (ribonucleoprotein)-based CRISPR/Cas9 strategy has recently been shown to be efficient in gene editing in diverse cell lines and organisms. Application of RNP strategy in salmon cell biology is still at its ... -
Exploring the Biotechnological Potential of the Secretome from the Marine Fungus Digitatispora marina Proteomics, Bioinformatics and Heterologous Expression of Novel Proteases
(Master thesis; Mastergradsoppgave, 2022-05-16)Cold-adapted enzymes are of biotechnological value due to their high catalytic powers at low to moderate temperatures and their high thermolability at elevated temperatures. Marine fungi are an understudied group of organisms, which makes them interesting as enzyme producers because of their adaptions to marine habitats. In this thesis study, a bioprospecting pipeline was constructed, and different ... -
The effect of microplastic on natural transformation and biofilm formation using Acinetobacter baylyi as a model organism
(Master thesis; Mastergradsoppgave, 2022-05-15)Microplastic pollution is a big and rapidly increasing environmental problem in the world. Although the direct effects of microplastic pollution are well-studied the indirect effects are hardly investigated, especially in the context of spreading antibiotic resistance genes. Antibiotic resistance is a natural phenomenon, but the misuse and overuse of antibiotics has led to a rapid development and ... -
Impact of CRISPR/Cas9 on the expressions of selected immune genes in salmonid cells
(Master thesis; Mastergradsoppgave, 2023-07-05)The discovery of CRISPR/Cas9 as a gene editing tool has revolutionized the field of molecular biology due to its huge potential for innovative applications. However, the biosafety concerns on CRISPR/Cas9-mediated gene editing like off-target mutations, triggering of cell death, and induction of cellular stress need to be elucidated. This thesis aims to investigate the impacts of electroporation ... -
Uptake of Polystyrene Nanoplastic and its Impacts on the Proteome of Salmonid cells
(Master thesis; Mastergradsoppgave, 2023-06-27)Micro- and nanoplastic particles, produced from the breakdown of larger plastic debris, represent a whole new field of hazardous pollution with the potential to cause great damage on a range of marine species. This escalating concern of plastic pollution in marine environments and its consequential impacts on marine species, has emphasized the urgency for an in-depth understanding of the uptake and ... -
Broad-Host-Range Genetic Tools for Observing Microbial Consortia
(Master thesis; Mastergradsoppgave, 2023-05-15)Microbial communities are complex assemblages that are key to ecosystem stability and human health. Synthetic ecology aims to design and construct microbial consortia with reduced complexity that enable innovative applications beyond monocultures. However, their robust and performance-based design is constrained by limited knowledge of growth dynamics and derived binary interactions that are critical ... -
Naphthoquinone pigments from the green sea urchin, Strongylocentrotus droebachiensis
(Master thesis; Mastergradsoppgave, 2021-02-08)Spinochromes are part of a subgroups of quinones called naphthoquinones. These molecules have a series of bioactivity making them a promising target for marine researchers all over the world. Sulphated spinochromes was recently identified, but their structure or bioactivity is yet to be elucidated. In this project, an attempt to extract these sulphated spinochromes from red spherule cells are made. ... -
Phototrophic, heterotrophic and mixotrophic growth in cold-adapted marine microalgae
(Master thesis; Mastergradsoppgave, 2022-05-15)Microalgae are grown in different habitats and conditions, such as high salinity, extreme temperatures, and variable pH. Cold-adapted microalgae may have the capacity of generating high biomass production, which can potentially be produced under cold and light-limited conditions in the Arctic. Most microalgae are obligated photoautotrophs; however, few species have been found living in complete ... -
Modeling the "microbial chassis effect" on the performance of a genetic switch
(Master thesis; Mastergradsoppgave, 2022-03-21)Escherichia coli is one of the most established bacterial hosts for genetic devices, partially due to the available knowledge and tools for ease of manipulation. However, there is an incentive to increase the current number of available recipients. For instance, marine bacteria are being recognized for their potential as microbial "chassis" due to their rich genetic and metabolic diversity. With ... -
Antimikrobielle forbindelser i kongsnegl (Buccinum undatum) og neptunsnegl (Neptunea despecta)
(Master thesis; Mastergradsoppgave, 2017-05-15)Det økende antall patogene bakteriestammer som utvikler resistens mot dagens kommersielle antibiotika er en av de største truslene mot den globale helsen i vår tid. Infeksjonssykdommer som tidligere kunne behandles med antibiotika har nå nesten blitt umulige å behandle, det er derfor et umiddelbart behov for nye typer antibiotika med nye virkningsmekanismer enn det som allerede eksisterer på markedet. ... -
Securidine analogues from the marine bryozoan Securiflustra securifrons.
(Master thesis; Mastergradsoppgave, 2021-05-12)Har isolert analoger av Securidine A, samt andre forbindelser fra arten Securiflustra securifrons, utført strukturoppklaring og testet for bioaktivitet. -
Assessing the effect of salmon aquaculture on fungal diversity in seawater and sediments through eDNA metabarcoding
(Master thesis; Mastergradsoppgave, 2021-05-16)Fungi have been found to play important roles in marine ecosystems, e.g., symbionts of plants and animals, and as decomposers of organic materials. The fungal communities, however, are often considered less studied and their functional and taxonomic diversity in aquatic environments remains poorly understood. Since the 1970s the Norwegian salmon industry developed from a small-scale industry to ... -
Manipulating the Epigenetic Machinery of Marine Fungi Through the Application of Epigenetic Modifiers to Induce the Biosynthesis of Novel Secondary Metabolites
(Master thesis; Mastergradsoppgave, 2020-05-15)Fungi are among the most prolific producers of secondary metabolites, many of which have huge utility for society as various anti-infective and anticancer drugs. The genes encoding these secondary metabolites are arranged in continuous biosynthetic gene clusters (BGCs). Each fungus can have tens of different BGCs in its genome, possessing the ability to produce a multitude of secondary metabolites ... -
Enzymatic protein hydrolysis of residual raw material from Atlantic cod: Selectivity of proteases, outcome and bioactivities
(Master thesis; Mastergradsoppgave, 2020-08-14)Global trends show that interest in fish products and fish consumption are increasing, while marine fishery resources are decreasing. Fish processing industry produces a high amount of residual raw materials that have nutritious proteins and other valuable compounds. An optimization of residual raw materials’ utilization can help meet the growing demand for fish products and help reduce environmental ... -
Chemical and Enzyme-Assisted Extraction of Fucoidan from two Species of Brown Macroalgae (Ascophyllum nodosum and Saccharina latissima)
(Master thesis; Mastergradsoppgave, 2021-05-18)Marine environments contain half of the global biodiversity and are a major source of bioactive compounds. Macroalgae are vital species in marine ecosystems and are known to produce several bioactive compounds and polysaccharides that possesses several bioactivities. These molecules are traditionally extracted using methods that heavily rely on hazardous solvents and heat, that more importantly ... -
Bioactivity evaluation of underutilized marine raw materials for the management of metabolic syndrome
(Master thesis; Mastergradsoppgave, 2021-05-15)Metabolic syndrome (MetS) is the medical term for a cluster of risk factors including obesity, high blood pressure, and high blood sugar, which increases the risk of developing stroke, heart disease, and type 2 diabetes. Marine raw materials have been described to exert various bioactive properties beneficial to human health, including anti-inflammatory, antioxidant, and antidiabetic abilities. In ...
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