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norskArtikler, rapporter og annet (medisinsk biologi): Recent submissions
Now showing items 281-300 of 1059
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Inhibition of bacterial and human zinc-metalloproteases by bisphosphonate- and catechol-containing compounds
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-23)Compounds containg catechol or bisphosphonate were tested as inhibitors of the zinc metalloproteases, thermolysin (TLN), pseudolysin (PLN) and aureolysin (ALN) which are bacterial virulence factors, and the human matrix metalloproteases MMP-9 and −14. Inhibition of virulence is a putative strategy in the development of antibacterial drugs, but the inhibitors should not interfere with human enzymes. ... -
Nephronectin as a matrix effector in cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-25)The extracellular matrix protein nephronectin plays an important regulatory role during embryonic development, controlling renal organogenesis through integrin α8β1 association. Nephronectin has three main domains: five N-terminal epidermal growth factor-like domains, a linker region harbouring two integrin-binding motifs (RGD and LFEIFEIER), and a C-terminal MAM domain. In this review, we look into ... -
Time trends of perfluoroalkyl substances in blood in 30-year old Norwegian men and women in the period 1986–2007
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-11)Biomonitoring studies are helpful tools and can increase our knowledge on time trends in human blood concentrations of PFASs: how they relate to emission trends and the potential prenatal exposure for future generations. In this study, serum was sampled in cross-sections of men and women who were 30 years old in each of the years 1986, 1994, 2001, and 2007 in Northern Norway and analyzed for 23 ... -
Evaluation of Merkel Cell Polyomavirus DNA in Tissue Samples from italian Patients With Diagnosis of MCC
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-05)Because the incidence of Merkel cell carcinoma (MCC) has increased significantly during the last 10 years and it is recognized that Merkel cell polyomavirus (MCPyV) and ultraviolet (UV) radiation represent two different etiological inputs sharing clinical, histopathological, and prognostic similar features, although with different prognosis, this study investigated the detection of MCPyV in skin and ... -
High prevalence of multidrug resistant ESBL- and plasmid mediated AmpC-producing clinical isolates of Escherichia coli at Maputo Central Hospital, Mozambique
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-06)Background: Epidemiological data of cephalosporin-resistant Enterobacterales in Sub-Saharan Africa is still restricted,and in particular in Mozambique. The aim of this study was to detect and characterize extended-spectrum β-lactamase (ESBL) - and plasmid-mediated AmpC (pAmpC)-producing clinical strains of Escherichia coli at Maputo Central Hospital (MCH), a 1000-bed reference hospital in Maputo, ... -
Real-life evaluation of histologic scores for Ulcerative Colitis in remission
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-08)Background - Histological evaluation of ulcerative colitis (UC) patients has been debated ever since the first description of the disease and its role in follow-up has never been fully established. Recent evidence suggests an added benefit in accuracy when evaluating if the patient is in remission. Unfortunately, there are several different histological indices, and it is difficult to compare outcomes ... -
KI and WU Polyomavirus in Respiratory Samples of SARS-CoV-2 Infected Patients
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-06-09)Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been declared a global pandemic. Our goal was to determine whether co-infections with respiratory polyomaviruses, such as Karolinska Institutet polyomavirus (KIPyV) and Washington University polyomavirus (WUPyV) occur in SARS-CoV-2 infected patients. Oropharyngeal swabs from 150 individuals, 112 symptomatic COVID-19 patients and 38 ... -
Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-10)Background - Heat-shock protein B1 (HSPB1) is among the most well-known and versatile member of the evolutionarily conserved family of small heat-shock proteins. It has been implicated to serve a neuroprotective role against various neurological disorders via its modulatory activity on inflammation, yet its exact role in neuroinflammation is poorly understood. In order to shed light on the exact ... -
Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-13)Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of ... -
Biodistribution of Poly(alkyl cyanoacrylate) Nanoparticles in Mice and Effect on Tumor Infiltration of Macrophages into a Patient-Derived Breast Cancer Xenograft
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-28)We have investigated the biodistribution and tumor macrophage infiltration after intravenous injection of the poly(alkyl cyanoacrylate) nanoparticles (NPs): PEBCA (poly(2-ethyl-butyl cyanoacrylate), PBCA (poly(n-butyl cyanoacrylate), and POCA (poly(octyl cyanoacrylate), in mice. These NPs are structurally similar, have similar PEGylation, and have previously been shown to give large variations in ... -
Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-24)<p>Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer and neurodegeneration. Autophagy-related 8 (ATG8) proteins play a central role in autophagosome formation and selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors and receptors. The LC3-interacting region (LIR) docking site (LDS) of ... -
Pre- and post-diagnostic blood profiles of chlorinated persistent organic pollutants and metabolic markers in type 2 diabetes mellitus cases and controls; a pilot study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-09)Objective - Several risk factors for type 2 diabetes mellitus (T2DM) are also associated with blood concentrations of persistent organic pollutants (POPs), and factors related to the disease may affect POP concentrations, and subsequent associations between POPs and T2DM. The purpose of this pilot study was to investigate the change in concentrations of lipids, hormones and POPs pre- and post-diagnosis ... -
Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-28)In total, 12 quinolone-resistant <i>Escherichia coli</i> (QREC) strains containing <i>qnrS1</i> were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly. -
The prevalence of HPA-1a alloimmunization and the potential risk of FNAIT depend on both the DRB3*01:01 allele and associated DR-DQ haplotypes
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-16)Alloimmunization against human platelet antigen (HPA)-1a during pregnancy can cause foetal/neonatal alloimmune thrombocytopenia (FNAIT) and severe bleeding in the foetus or newborn and likely depends on several factors. HPA-1a alloimmunization is associated with <i>DRB3*01:01</i>, which is associated with several DR-DQ haplotypes. However, it is not known to what extent these haplotypes contribute ... -
Genetic Diversity of the Noncoding Control Region of the Novel Human Polyomaviruses
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-07)The genomes of polyomaviruses are characterized by their tripartite organization with an early region, a late region and a noncoding control region (NCCR). The early region encodes proteins involved in replication and transcription of the viral genome, while expression of the late region generates the capsid proteins. Transcription regulatory sequences for expression of the early and late genes, as ... -
Hemodynamic effects of a soluble guanylate cyclase stimulator, Riociguat, and an activator, Cinaciguat, during NO-modulation in healthy pigs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-14)Cardiovascular diseases are often characterized by dysfunctional endothelium. To compensate for the related lack of nitric oxide (NO), a class of soluble guanylate cyclase (sGC) stimulators and activators have been developed with the purpose of acting downstream of NO in the NO-sGC-cGMP cascade. These drugs have been discovered using photoaffinity labeling of sGC and high-throughput screening of a ... -
Prognostic value of absolute quantification of mutated KRAS in circulating tumour DNA in lung adenocarcinoma patients prior to therapy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-27)Droplet digital polymerase chain reaction (ddPCR) is a highly sensitive and accurate method for quantification of nucleic acid sequences. We used absolute quantification of mutated v‐Ki‐ras2 Kirsten rat sarcoma viral oncogene homology gene (KRAS) by ddPCR to investigate the prognostic role of mutated KRAS in patients with KRAS‐mutated lung adenocarcinomas. Pre‐treatment plasma samples from 60 patients ... -
Tumor-associated macrophages and macrophage-related immune checkpoint expression in sarcomas
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-12)Early trials for immune checkpoint inhibitors in sarcomas have delivered mixed results, and efforts to improve outcomes now look to combinatorial strategies with novel immunotherapeutics, including some that target macrophages. To enhance our understanding of the sarcoma immune landscape, we quantified and characterized tumor-associated macrophage infiltration and expression of the targetable ... -
Exploring Conformational Dynamics of the Extracellular Venus flytrap Domain of the GABA B Receptor: A Path-Metadynamics Study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-01)γ-Aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the central nervous system (CNS). Dysfunctional GABAergic neurotransmission is associated with numerous neurological and neuropsychiatric disorders. The GABA<sub>B</sub> receptor (GABA<sub>B</sub>-R) is a heterodimeric class C G protein-coupled receptor (GPCR) comprised of GABA<sub>B1a/b</sub> and GABA<sub>B2</sub> subunits. The ... -
Kidney tertiary lymphoid structures in Lupus Nephritis develop into large interconnected networks and resemble lymph nodes in gene signature
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-16)Immune aggregates organized as tertiary lymphoid structures (TLS) are observed within the kidneys of patients with systemic lupus erythematosus and lupus nephritis (LN). Renal TLS was characterized in lupus-prone New Zealand black × New Zealand white F1 mice analyzing cell composition and vessel formation. RNA sequencing was performed on transcriptomes isolated from lymph nodes, macrodissected TLS ...
