English
norskArtikler, rapporter og annet (medisinsk biologi): Recent submissions
Now showing items 441-460 of 1059
-
In silico site-directed mutagenesis of the Daphnia magna ecdysone receptor identifies critical amino acids for species-specific and inter-species differences in agonist binding
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-04)Molting is an essential process in the life cycle of arthropods and is regulated by complex neuroendocrine pathways where activation of the ecdysone receptor (EcR) plays a major role. The EcR forms a non-covalent heterodimer with the ultraspiracle protein (USP) when activated by endogenous ecdysteroids, but can also be activated by several insecticides and other environmental chemicals. Environmental ... -
Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-10)<i>Background</i> - Blood culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo blood culture system for culturing periprosthetic tissue specimens. <p> ... -
Prognostic biomarkers for immunotherapy with ipilimumab in metastatic melanoma
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-01)New therapies, including the anti‐cytotoxic T lymphocyte antigen (CTLA)‐4 antibody, ipilimumab, is approved for metastatic melanoma. Prognostic biomarkers need to be identified, because the treatment has serious side effects. Serum samples were obtained before and during treatment from 56 patients with metastatic or unresectable malignant melanoma, receiving treatment with ipilimumab in a national ... -
Novel targets for delaying aging: The importance of the liver and advances in drug delivery
(Journal article; Tidsskriftartikkel, 2018-09-21)Age-related changes in liver function have a significant impact on systemic aging and susceptibility to age-related diseases. Nutrient sensing pathways have emerged as important targets for the development of drugs that delay aging and the onset age-related diseases. This supports a central role for the hepatic regulation of metabolism in the association between nutrition and aging. Recently, a role ... -
In Silico Methods for the Discovery of Orthosteric GABAB Receptor Compounds
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-07)The GABA<sub>B</sub> receptor (GABA<sub>B</sub>-R) is a heterodimeric class C G protein-coupled receptor comprised of the GABA<sub>B1a/b</sub> and GABA<sub>B2</sub> subunits. The endogenous orthosteric agonist γ-amino-butyric acid (GABA) binds within the extracellular Venus flytrap (VFT) domain of the GABA<sub>B1a/b</sub> subunit. The receptor is associated with numerous neurological and ... -
A Three-dimensional Ex Vivo Viability Assay Reveals a Strong Correlation Between Response to Targeted Inhibitors and Mutation Status in Melanoma Lymph Node Metastases
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-13)Although clinical management of melanoma has changed considerably in recent years, intrinsic treatment resistance remains a severe problem and strategies to design personal treatment regimens are highly warranted. We have applied a three-dimensional (3D) <i>ex vivo</i> drug efficacy assay, exposing disaggregated cells from 38 freshly harvested melanoma lymph node metastases and 21 patient derived ... -
Exploring the overlapping binding sites of ifenprodil and EVT‐101 in GluN2B‐containing NMDA receptors using novel chicken embryo forebrain cultures and molecular modeling
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-30)N‐methyl‐d‐aspartate receptors (NMDAR) are widely expressed in the brain. GluN2B subunit‐containing NMDARs has recently attracted significant attention as potential pharmacological targets, with emphasis on the functional properties of allosteric antagonists. We used primary cultures from chicken embryo forebrain (E10), expressing native GluN2B‐containing NMDA receptors as a novel model system. ... -
MicroRNA 141 is associated to outcome and aggressive tumor characteristics in prostate cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-23)A large number of miRNAs influence key cellular processes involved in prostate tumorigenesis. Previous studies have demonstrated high expression of miRNAs in human prostate cancer (PC) tissues and cell lines. In previous microarray data, we found miR-141 to be upregulated and miR-145 to be downregulated in PC. In this large PC cohort (n = 535), we explored the prognostic role of miR-141 and miR-145 ... -
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viridis
(Journal article; Peer reviewed, 2019-05-08)Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied animals, such as sea anemones, are often resilient. We sequenced the polyA-enriched transcriptome of adult sea anemone Anemonia viridis and its dinoflagellate symbiont sampled along ... -
The long noncoding RNA NEAT1 and nuclear paraspeckles are up-regulated by the transcription factor HSF1 in the heat shock response
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-10)The long noncoding RNA (lncRNA) <i>NEAT1</i> (nuclear enriched abundant transcript 1) is the architectural component of nuclear paraspeckles, and it has recently gained considerable attention as it is abnormally expressed in pathological conditions such as cancer and neurodegenerative diseases. <i>NEAT1</i> and paraspeckle formation are increased in cells upon exposure to a variety of environmental ... -
Screening for hepatitis C in a general adult population in a low-prevalence area: the Tromsø study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-26)<i>Background - </i>Chronic hepatitis C virus (HCV) infection can progress to cirrhosis and end-stage liver disease in a substantial proportion of patients. The infection is frequently asymptomatic, leaving many infected individuals unaware of the diagnosis until complications occur. This advocates the screening of healthy individuals. The aim of this study was to estimate the prevalence of HCV ... -
O-GlcNAc Transferase Inhibition Differentially Affects Breast Cancer Subtypes
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-04-05)Post-translational modifcation of intracellular proteins with a single N-acetylglucosamine sugar (O-GlcNAcylation) regulates signaling, proliferation, metabolism and protein stability. In breast cancer, expression of the enzyme that catalyzes O-GlcNAcylation – O-GlcNAc-transferase (OGT), and the extent of protein O-GlcNAcylation, are upregulated in tumor tissue, and correlate with cancer progression. ... -
Discordance, Disclosure and Normative Gender Roles: Barriers to Couple Testing Within a Community-Level HIV Self-Testing Intervention in Urban Blantyre, Malawi
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-06)A community-based HIV self-testing study in Blantyre, Malawi demonstrated that not all individuals living in couples tested with their partner. We describe factors dissuading individuals in couples from self-testing with their partner. Data were drawn from qualitative study exploring consequences of HIV self-testing within couples. In-depth interviews were conducted with 33 individuals living in ... -
Update on prevalence and mechanisms of resistance to linezolid, tigecycline and daptomycin in enterococci in Europe: towards a common nomenclature
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-02)Vancomycin-resistant enterococci (VRE) are important nosocomial pathogens. Invasive VRE infections are difficult to treat since common therapeutic options including ampicillin and glycopeptides often fail. In vitro, most VRE remain susceptible to last-resort antibiotics such as linezolid, tigecycline and daptomycin. However, neither tigecycline nor linezolid act in a bactericidal manner, and ... -
Risk perception, beliefs about medicines and medical adherence among pregnant and breastfeeding women with migraine: findings from a cross-sectional study in Norway
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-27)<i>Objectives</i>: To examine risk perception, beliefs about migraine medications and medical adherence among pregnant and breastfeeding women with migraine.<p> <p><i>Design and setting</i>: Cross-sectional study conducted in Norway from October 2013 to February 2014. Data were collected via an anonymous, electronic questionnaire.<p> <p><i>Participants</i>: Women with migraine, either ... -
Myocardial NADPH oxidase-4 regulates the physiological response to acute exercise
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-12-27)Regular exercise has widespread health benefits. Fundamental to these beneficial effects is the ability of the heart to intermittently and substantially increase its performance without incurring damage, but the underlying homeostatic mechanisms are unclear. We identify the ROSgenerating NADPH oxidase-4 (Nox4) as an essential regulator of exercise performance in mice. Myocardial Nox4 levels ... -
Thrombospondin-1 Is Necessary for the Development and Repair of Corneal Nerves
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-16)Thrombospondin-1-deficient (TSP-1−/−) mice are used as an animal model of Sjögren’s Syndrome because they exhibit many of the symptoms associated with the autoimmune type of dry eye found in primary Sjögren’s Syndrome. This type of dry eye is linked to the inflammation of the lacrimal gland, conjunctiva, and cornea, and is thought to involve dysfunction of the complex neuronal reflex arc that ... -
Normalization of disrupted clock gene expression in males with tetraplegia: a crossover randomized placebo-controlled trial of melatonin supplementation
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-09)<i>Study design</i>: Crossover double blind, randomized placebo-controlled trial.<p> <p><i>Objectives</i>: Circadian oscillators are located both in the brain and in peripheral organs. Melatonin, the main brain-derived hormone governing circadian variations, is highly associated with daylight patterns. However, in subjects with tetraplegia the melatonin levels are blunted. Here we studied peripheral ... -
Starvation induces rapid degradation of selective autophagy receptors by endosomal microautophagy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-17)It is not clear to what extent starvation-induced autophagy affects the proteome on a global scale and whether it is selective. In this study, we report based on quantitative proteomics that cells during the first 4 h of acute starvation elicit lysosomal degradation of up to 2–3% of the proteome. The most significant changes are caused by an immediate autophagic response elicited by shortage of amino ... -
Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-23)Abstract Oncolytic peptides and peptidomimetics are being optimized for the treatment of cancer by selecting agents with high cytotoxic potential to kill a maximum of tumor cells as well as the capacity to trigger anticancer immune responses and hence to achieve long-term effects beyond therapeutic discontinuation. Here, we report on the characterization of two novel oncolytic peptides, DTT-205 ...
