Browsing Institutt for farmasi by Title
Now showing items 138-157 of 652
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Dacarbazine and the Agonistic TRAIL Receptor-2 Antibody Lexatumumab Induce Synergistic Anticancer Effects in Melanoma
(Journal article; Tidsskriftartikkel; Peer reviewed, 2012)Mapatumumab and lexatumumab (targeting death receptor 4 (DR4) and 5 (DR5), respectively) are agonistic TRAIL receptor antibodies that induce apoptosis in a wide range of cancer cells. The potency of mapatumumab and lexatumumab was assessed in mono therapy protocols, and the ability to sensitize for dacarbazine (DTIC) treatment was explored in ten different melanoma cell lines. Our data indicated ... -
Deciphering the landscape of host barriers to Listeria monocytogenes infection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-05-30)<i>Listeria monocytogenes</i> is a common food-borne pathogen that can disseminate from the intestine and infect multiple organs. Here, we used sequence tag-based analysis of microbial populations (STAMP) to investigate <i>L. monocytogenes</i> population dynamics during infection. We created a genetically barcoded library of murinized <i>L. monocytogenes</i> and then used deep sequencing to track ... -
Deformable liposomes for skin therapy with human epidermal growth factor: The effect of liposomal surface charge
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-07)The topical administration of exogenous human epidermal growth factor (hEGF) is a promising approach for improved chronic wound therapy. To develop therapeutically superior hEGF formulation, we prepared hEGF-containing neutral (NDLs), cationic (CDLs) and anionic (ADLs) deformable liposomes (DLs), respectively, since it is expected that the liposomal surface charge can affect both the liposomal ... -
Dermal Application of Chloramphenicol - The Effect of Liposomes and Chitosan Hydrogel Formulations on ex vivo Permeation and Antimicrobial Activity
(Conference object; Konferansebidrag, 2017)Liposomes solubilize lipophilic drugs in the phospholipid membrane, and entrap hydrophilic drugs in its aqueous core. These vesicles provide a sustained- and targeted- drug release, and protect from degradation. Liposomes retain the drug onto/in the skin and improve skin drug deposition. Due to their liquid nature, a secondary vehicle, such as a hydrogel, is needed to obtain a necessary retention ... -
Design and synthesis of novel cyclopentapeptide antagonist for the chemokine receptor CXCR4
(Master thesis; Mastergradsoppgave, 2008-06-16)CXCR4 is a GPCR that by activation of its ligand CXCL12 is involved in the pathology of several diseases, examples being cancer and rheumatoid arthritis. It has also shown to play a crucial role for HIV-1 entry into T-cells and the development of AIDS. Several cyclopentapeptides (CPPs) based on the sequence D-Tyr-Arg-Arg-2-Nal-Gly of the lead compound FC131 have shown to have antagonistic activity. ... -
Design og syntese av cyklopentapeptidbaserte CXCR4 antagonister: Variasjon av essensiell aromatisk substituent
(Mastergradsoppgave; Master thesis, 2009-05-20)SAMMENDRAG CXCR4 er en kjemokinreseptor som er involvert i en rekke biologiske prosesser i kroppen, blant annet angiogenese, neurogenese og dannelse av blodceller i embryostadiet. I voksne er CXCR4 funnet å være involvert i sykdommer som HIV, reumatoid artritt og kreft. I litteraturen finnes det potente CXCR4 antagonister (AMD3100, KRH1636, FC131) som har vist effekt mot HIV-1, reumatoid artritt ... -
Design, synthesis and structural studies of relaxin receptor modulators
(Master thesis; Mastergradsoppgave, 2011-06)The relaxin family of peptides consists of seven structurally related peptides, with a wide variety of biological functions, ranging from involvement in reproductive processes to functions as neuroendocrine modulators. Because of the complex, two-chained structure of the relaxins, and their lack of specificity for the various relaxin family receptors (RXFPs), design of simpler, more selective ... -
Design, synthesis, and biological evaluation of scaffold-based tripeptidomimetic antagonists for CXC chemokine receptor 4 (CXCR4)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014-07-14) -
Det metastaseassosierte proteinet S100A4 i orale plateepitelkarsinomcellelinjer - kartlegging av effekter på proteolytiske enzymer viktige for invasjon og metastase
(Master thesis; Mastergradsoppgave, 2012-05-20)Oralkreft er en kreftform forbundet med dårlig prognose, som hovedsakelig skyldes hyppig forekomst av lokal invasjon og metastase til lymfeknuter. Behandlingen består i dag hovedsakelig av kirurgi og stråling, men på tross av stadig nye og bedre behandlingsformer er mortaliteten for pasienter med oralkreft uendret de siste 30 årene. Med den vedvarende høye dødsraten av oralkreft er det et tydelig ... -
Detecting rare gene transfer events in bacterial populations
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Detection of carbapenemases with a newly developed commercial assay using Matrix Assisted Laser Desorption Ionization-Time of Flight
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-03)This study evaluated the performance of the MBT STAR-Carba kit (Bruker Daltonics), to detect carbapenemase producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. in comparison with the RAPIDEC® CARBA NP test (BioMerieux). MBT STAR-Carba allowed the detection of carbapenemases in Enterobacteriaceae and P. aeruginosa. -
Detection of DHCMT long-term metabolite glucuronides with LC-MSMS as an alternative approach to conventional GC-MSMS analysis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-17)Dehydrochloromethyltestosterone (DHCMT) is one of the most detected illicit used anabolic–androgenic steroids in professional sports. Therefore, a fast and accurate analysis of this substance is of great importance for a constructive fight against doping abuse. The conventional method for the analysis of this drug, GC-MSMS, is very sensitive and selective but also very time- and resource-consuming. ... -
Detection of disseminated tumor cells in lymph nodes from patients with early stage non-small cell lung cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-06-17)Background: The regional lymph node involvement is a major prognostic factor in patients with non-small cell lung cancer (NSCLC) undergoing surgical resection. Disease relapse is common, suggesting that early disseminated disease is already present in the regional lymph nodes at the time of surgery, and that the current nodal staging classification might be suboptimal. Early detection of disseminated ... -
Determination of Cysteine Connectivity in Bioactive Peptides
(Master thesis; Mastergradsoppgave, 2014-05-20)Conotoxins are diverse and well-studied, and is therefore suited as a model to develop a general method for analyzing disulfide-rich peptides. To investigate the folding of both commercially available and self-synthesized Conotoxins, an UHPLC-MS/MS method was developed where one disulfide-bridge was reduced at the time and alkylated with different maleimides. The results from the UHPLC-MS/MS method ... -
Determination of the binding mode for the cyclopentapeptide CXCR4 antagonist FC131 using a dual approach of ligand modifications and receptor mutagenesis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Developement of mucoadhesive polymer-coated liposomes for hydration of the oral mucosa
(Master thesis; Mastergradsoppgave, 2013-05-18)Dry mucus in the oral cavity is uncomfortable for patients and can lead to other additional illnesses. Reduced or absence of saliva secretion, caused by the diseases, side effects of drugs, aging, or radiotherapy, can result in issues related to xerostomia and dental health. A system that could contribute to increased hydration of the mucus would reduce the discomfort for the patient. In addition, ... -
Development and evaluation of a test program for Y-site compatibility testing of total parenteral nutrition and intravenous drugs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-03-22)Background: There is no standardized procedure or consensus to which tests should be performed to judge compatibility/ incompatibility of intravenous drugs. The purpose of this study was to establish and evaluate a test program of methods suitable for detection of physical incompatibility in Y-site administration of total parenteral nutrition (TPN) and drugs. Methods: Eight frequently used methods ... -
Development and optimization of a new processing approach for manufacturing topical liposomes-in-hydrogel drug formulations by dual asymmetric centrifugation
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-01-24)Objectives: The objective of the present study was to utilize dual asymmetric centrifugation (DAC) as a novel processing approach for the production of liposomes-in-hydrogel formulations. <br>Materials and Methods: Lipid films of phosphatidylcholine, with and without chloramphenicol (CAM), were hydrated and homogenized by DAC to produce liposomes in the form of vesicular phospholipid gels with a ... -
Development and validation of a simple online-SPE method coupled to high-resolution mass spectrometry for the analysis of stanozolol-N-glucuronides in urine samples
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-17)Stanozolol is still the most commonly used illicit anabolic‐androgenic steroid (AAS) in professional sports. Therefore, accurate and fast analysis and long detection windows are of great interest in the field of antidoping analysis. In this work, a very simple, fast, and highly sensitive online solid‐phase extraction method coupled with liquid chromatography–high‐resolution tandem mass spectrometry ... -
Development of a biomimetic phospholipid vesicle-based permeation assay for the estimation of intestinal drug permeability
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014)